- SPINE (molecular biology)
SPINE stands for Strep-Protein INteraction Experiment and serves in the detection of
protein-protein interaction s "in vivo". A protein is tagged with a Strep-tag and is expressed in the cell under the conditions where interaction partners are searched. An addition offormaldehyde links the protein to its interaction partners. The complex can then be isolated with a Streptactin column. Since the cross-linking of the proteins with formaldehyde is reversible after heating, the interaction partners of the tagged protein can be detected in anSDS-PAGE .References
* Herzberg C., Weidinger LA., Dörrbecker B., Hübner S., Stülke J. and Commichau FM (2007). [http://www3.interscience.wiley.com/cgi-bin/abstract/116841233/ABSTRACT SPINE: A method for the rapid detection and analysis of protein-protein interactions "in vivo"] . "Proteomics" 7(22):4032-4035
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