DDOST

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase
Identifiers
Symbols	DDOST; AGER1; KIAA0115; MGC2191; OKSWcl45; OST; OST48; WBP1
External IDs	OMIM: 602202 MGI: 1194508 HomoloGene: 3821 GeneCards: DDOST Gene
EC number	2.4.1.119
Gene Ontology Molecular function • dolichyl-diphosphooligosaccharide-protein glycotransferase activity • dolichyl-diphosphooligosaccharide-protein glycotransferase activity • protein binding • transferase activity Cellular component • endoplasmic reticulum • endoplasmic reticulum membrane • microsome • oligosaccharyltransferase complex • membrane • integral to membrane • protein complex Biological process • protein N-linked glycosylation • protein N-linked glycosylation via asparagine • protein N-linked glycosylation via asparagine • response to cytokine stimulus • T cell activation • post-translational protein modification • cellular protein metabolic process • innate immune response Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	1650	13200
Ensembl	ENSG00000244038	ENSMUSG00000028757
UniProt	P39656	Q3UC51
RefSeq (mRNA)	NM_005216	NM_007838.2
RefSeq (protein)	NP_005207	NP_031864.2
Location (UCSC)	Chr 1: 20.98 – 20.99 Mb	Chr 4: 137.86 – 137.87 Mb
PubMed search	[1]	[2]

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit is an enzyme that in humans is encoded by the DDOST gene.^[1][2]

This gene encodes a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. The product of this gene is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia.^[2]

References

1. ^ Yamagata T, Tsuru T, Momoi MY, Suwa K, Nozaki Y, Mukasa T, Ohashi H, Fukushima Y, Momoi T (Jan 1998). "Genome organization of human 48-kDa oligosaccharyltransferase (DDOST)". Genomics 45 (3): 535–40. doi:10.1006/geno.1997.4966. PMID 9367678. 
2. ^ ^{a b} "Entrez Gene: DDOST dolichyl-diphosphooligosaccharide-protein glycosyltransferase". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=1650. 

Further reading

• Kumar V, Korza G, Heinemann FS, Ozols J (1995). "Human oligosaccharyltransferase: isolation, characterization, and the complete amino acid sequence of 50-kDa subunit". Arch. Biochem. Biophys. 320 (2): 217–23. doi:10.1016/0003-9861(95)90003-9. PMID 7625827. 
• Nagase T, Miyajima N, Tanaka A, et al. (1995). "Prediction of the coding sequences of unidentified human genes. III. The coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by analysis of cDNA clones from human cell line KG-1". DNA Res. 2 (1): 37–43. doi:10.1093/dnares/2.1.37. PMID 7788527. 
• Stitt AW, He C, Vlassara H (1999). "Characterization of the advanced glycation end-product receptor complex in human vascular endothelial cells". Biochem. Biophys. Res. Commun. 256 (3): 549–56. doi:10.1006/bbrc.1999.0291. PMID 10080935. 
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241. 
• Lu C, He JC, Cai W, et al. (2004). "Advanced glycation endproduct (AGE) receptor 1 is a negative regulator of the inflammatory response to AGE in mesangial cells". Proc. Natl. Acad. Sci. U.S.A. 101 (32): 11767–72. doi:10.1073/pnas.0401588101. PMC 511050. PMID 15289604. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=511050. 
• Suzuki Y, Yamashita R, Shirota M, et al. (2004). "Sequence Comparison of Human and Mouse Genes Reveals a Homologous Block Structure in the Promoter Regions". Genome Res. 14 (9): 1711–8. doi:10.1101/gr.2435604. PMC 515316. PMID 15342556. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=515316. 
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928. 
• Shibatani T, David LL, McCormack AL, et al. (2005). "Proteomic analysis of mammalian oligosaccharyltransferase reveals multiple subcomplexes that contain Sec61, TRAP, and two potential new subunits". Biochemistry 44 (16): 5982–92. doi:10.1021/bi047328f. PMID 15835887. 
• Ewing RM, Chu P, Elisma F, et al. (2007). "Large-scale mapping of human protein–protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1847948.