Unspecific monooxygenase

Unspecific monooxygenase
unspecific monooxygenase
EC number
CAS number 62213-32-5
IntEnz IntEnz view
ExPASy NiceZyme view
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum
Gene Ontology AmiGO / EGO

In enzymology, an unspecific monooxygenase (EC is an enzyme that catalyzes the chemical reaction

RH + reduced flavoprotein + O2 \rightleftharpoons ROH + oxidized flavoprotein + H2O

The 3 substrates of this enzyme are RH, reduced flavoprotein, and O2, whereas its 3 products are ROH, oxidized flavoprotein, and H2O.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor. The systematic name of this enzyme class is substrate,reduced-flavoprotein:oxygen oxidoreductase (RH-hydroxylating or -epoxidizing). Other names in common use include microsomal monooxygenase, xenobiotic monooxygenase, aryl-4-monooxygenase, aryl hydrocarbon hydroxylase, microsomal P-450, flavoprotein-linked monooxygenase, and flavoprotein monooxygenase. This enzyme participates in 7 metabolic pathways: fatty acid metabolism, androgen and estrogen metabolism, gamma-hexachlorocyclohexane degradation, tryptophan metabolism, arachidonic acid metabolism, linoleic acid metabolism, and metabolism of xenobiotics by cytochrome p450. It employs one cofactor, heme.

Structural studies

As of late 2007, 53 structures have been solved for this class of enzymes, with PDB accession codes 1BU7, 1BVY, 1DT6, 1FAG, 1FAH, 1JME, 1JPZ, 1N6B, 1NR6, 1OG2, 1OG5, 1P0V, 1P0W, 1P0X, 1PO5, 1PQ2, 1R9O, 1SMI, 1SMJ, 1SUO, 1TQN, 1W0E, 1W0F, 1W0G, 1YQO, 1YQP, 1Z10, 1Z11, 1ZO4, 1ZO9, 1ZOA, 2BDM, 2BMH, 2F9Q, 2FDU, 2FDV, 2FDW, 2FDY, 2HI4, 2HPD, 2IJ2, 2IJ3, 2IJ4, 2J0D, 2J1M, 2J4S, 2NNB, 2P85, 2PG5, 2PG6, 2PG7, 2UWH, and 2V0M.


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