Top-down proteomics

Top-down proteomics

Top-down proteomics is a method of protein identification that uses an ion trapping mass spectrometer to store an isolated protein ion for mass measurement and tandem mass spectrometry analysis.cite journal |author=Sze SK, Ge Y, Oh H, McLafferty FW |title=Top-down mass spectrometry of a 29-kDa protein for characterization of any posttranslational modification to within one residue |journal=Proc. Natl. Acad. Sci. U.S.A. |volume=99 |issue=4 |pages=1774–9 |year=2002 |pmid=11842225 |doi=10.1073/pnas.251691898 |issn=] cite journal |author=Kelleher NL |title=Top-down proteomics |journal=Anal. Chem. |volume=76 |issue=11 |pages=197A–203A |year=2004 |pmid=15190879 |doi= |issn=] The name is derived from the similar approach to DNA seqencing.cite journal |author=Smith CL, Cantor CR |title=Evolving strategies for making physical maps of mammalian chromosomes |journal=Genome |volume=31 |issue=2 |pages=1055–8 |year=1989 |pmid=2698822 |doi= |issn=] Proteins are typically ionized by electrospray ionization and trapped in a Fourier transform ion cyclotron resonance (Penning trap)cite journal |author=Bogdanov B, Smith RD |title=Proteomics by FTICR mass spectrometry: top down and bottom up |journal=Mass spectrometry reviews |volume=24 |issue=2 |pages=168–200 |year=2005 |pmid=15389855 |doi=10.1002/mas.20015 |issn=] or quadrupole ion trap (Paul trap) mass spectrometer. Fragmentation for tandem mass spectrometry is accomplished by electron capture dissociation or electron transfer dissociation.

ee also

*Protein mass spectrometry
*Bottom-up proteomics
*Shotgun proteomics

References

Bibliography

*cite journal |author=Borchers CH, Thapar R, Petrotchenko EV, "et al" |title=Combined top-down and bottom-up proteomics identifies a phosphorylation site in stem-loop-binding proteins that contributes to high-affinity RNA binding |journal=Proc. Natl. Acad. Sci. U.S.A. |volume=103 |issue=9 |pages=3094–9 |year=2006 |pmid=16492733 |doi=10.1073/pnas.0511289103
*cite journal |author=Han X, Jin M, Breuker K, McLafferty FW |title=Extending top-down mass spectrometry to proteins with masses greater than 200 kilodaltons |journal=Science |volume=314 |issue=5796 |pages=109–12 |year=2006 |pmid=17023655 |doi=10.1126/science.1128868
*cite journal |author=Whitelegge J, Halgand F, Souda P, Zabrouskov V |title=Top-down mass spectrometry of integral membrane proteins |journal=Expert review of proteomics |volume=3 |issue=6 |pages=585–96 |year=2006 |pmid=17181473 |doi=10.1586/14789450.3.6.585 |issn=


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