Top-down proteomics

Top-down proteomics

Top-down proteomics is a method of protein identification that uses an ion trapping mass spectrometer to store an isolated protein ion for mass measurement and tandem mass spectrometry analysis.cite journal |author=Sze SK, Ge Y, Oh H, McLafferty FW |title=Top-down mass spectrometry of a 29-kDa protein for characterization of any posttranslational modification to within one residue |journal=Proc. Natl. Acad. Sci. U.S.A. |volume=99 |issue=4 |pages=1774–9 |year=2002 |pmid=11842225 |doi=10.1073/pnas.251691898 |issn=] cite journal |author=Kelleher NL |title=Top-down proteomics |journal=Anal. Chem. |volume=76 |issue=11 |pages=197A–203A |year=2004 |pmid=15190879 |doi= |issn=] The name is derived from the similar approach to DNA seqencing.cite journal |author=Smith CL, Cantor CR |title=Evolving strategies for making physical maps of mammalian chromosomes |journal=Genome |volume=31 |issue=2 |pages=1055–8 |year=1989 |pmid=2698822 |doi= |issn=] Proteins are typically ionized by electrospray ionization and trapped in a Fourier transform ion cyclotron resonance (Penning trap)cite journal |author=Bogdanov B, Smith RD |title=Proteomics by FTICR mass spectrometry: top down and bottom up |journal=Mass spectrometry reviews |volume=24 |issue=2 |pages=168–200 |year=2005 |pmid=15389855 |doi=10.1002/mas.20015 |issn=] or quadrupole ion trap (Paul trap) mass spectrometer. Fragmentation for tandem mass spectrometry is accomplished by electron capture dissociation or electron transfer dissociation.

ee also

*Protein mass spectrometry
*Bottom-up proteomics
*Shotgun proteomics

References

Bibliography

*cite journal |author=Borchers CH, Thapar R, Petrotchenko EV, "et al" |title=Combined top-down and bottom-up proteomics identifies a phosphorylation site in stem-loop-binding proteins that contributes to high-affinity RNA binding |journal=Proc. Natl. Acad. Sci. U.S.A. |volume=103 |issue=9 |pages=3094–9 |year=2006 |pmid=16492733 |doi=10.1073/pnas.0511289103
*cite journal |author=Han X, Jin M, Breuker K, McLafferty FW |title=Extending top-down mass spectrometry to proteins with masses greater than 200 kilodaltons |journal=Science |volume=314 |issue=5796 |pages=109–12 |year=2006 |pmid=17023655 |doi=10.1126/science.1128868
*cite journal |author=Whitelegge J, Halgand F, Souda P, Zabrouskov V |title=Top-down mass spectrometry of integral membrane proteins |journal=Expert review of proteomics |volume=3 |issue=6 |pages=585–96 |year=2006 |pmid=17181473 |doi=10.1586/14789450.3.6.585 |issn=


Wikimedia Foundation. 2010.

Игры ⚽ Поможем сделать НИР

Look at other dictionaries:

  • Top-down (disambiguation) — Top down may refer to:In systems theory, engineering and software: * Top down and bottom up design * Top down parsingIn computer games: * Top down perspective, a camera angle in computer and video games * Top down shooter, the computer and video… …   Wikipedia

  • Proteomics — is the large scale study of proteins, particularly their structures and functions.cite journal |author=Anderson NL, Anderson NG |title=Proteome and proteomics: new technologies, new concepts, and new words |journal=Electrophoresis |volume=19… …   Wikipedia

  • Bottom-up proteomics — is a common method to identify proteins and characterize their amino acid sequences and post translational modifications by proteolytic digestion of proteins prior to analysis by mass spectrometry.cite journal |author=Aebersold R, Mann M… …   Wikipedia

  • Shotgun proteomics — is a method of identifying proteins in complex mixtures using a combination of high performance liquid chromatography combined with mass spectrometry.cite journal |author=Hu L, Ye M, Jiang X, Feng S, Zou H |title=Advances in hyphenated analytical …   Wikipedia

  • Neil Kelleher (scientist) — Neil L. Kelleher Residence United States Nationality United States Fields …   Wikipedia

  • Tandem mass spectrometry — Tandem mass spectrometry, also known as MS/MS, involves multiple steps of mass spectrometry selection, with some form of fragmentation occurring in between the stages. [IUPAC gold book definition of tandem mass spectrometer… …   Wikipedia

  • Peptide mass fingerprinting — Citation maintenance. Please you see. Any concerns? Please . Peptide mass fingerprinting (PMF) (also known as protein fingerprinting) is an analytical technique for protein identification that was developed in 1993 by several groups independently …   Wikipedia

  • Electron transfer dissociation — (ETD) is a method to fragment ions in a mass spectrometer.cite journal |author=Syka JE, Coon JJ, Schroeder MJ, Shabanowitz J, Hunt DF |title=Peptide and protein sequence analysis by electron transfer dissociation mass spectrometry |journal=Proc.… …   Wikipedia

  • Enterohämorrhagische Escherichia coli — Klassifikation nach ICD 10 A04 Sonstige bakterielle Darminfektionen A04.3 Darminfektion durch enterohämorrhagische Escherichia coli …   Deutsch Wikipedia

  • Chemical biology — is a scientific discipline spanning the fields of chemistry and biology that involves the application of chemical techniques and tools, often compounds produced through synthetic chemistry, to the study and manipulation of biological systems.… …   Wikipedia

Share the article and excerpts

Direct link
Do a right-click on the link above
and select “Copy Link”