DNA construct

DNA construct

A DNA construct (stress on first syllable) is an artificially constructed segment of nucleic acid that is going to be "transplanted" into a target tissue or cell. It often contains a DNA insert, which contains the gene sequence encoding a protein of interest, that has been subcloned into a vector, which contains bacterial resistance genes for growth in bacteria, and promoters for expression in the organism. A DNA construct may express wildtype protein, prevent the expression of certain genes by expressing competitors or inhibitors, or express mutant proteins, such as deletion mutations or missense mutations. A DNA construct is often used in molecular biology to analyze macromolecules such as proteins or RNA in more detail.

See also

  • Rescue construct
  • Disruption construct



Wikimedia Foundation. 2010.

Игры ⚽ Нужно сделать НИР?

Look at other dictionaries:

  • DNA construct — A chimeric DNA molecule, carrying all the genetic information necessary for its transgenic expression in a host cell …   Glossary of Biotechnology

  • DNA-encoded chemical library — DNA encoded chemical libraries (DEL) are a new technology for the synthesis and screening of collections of chemical compounds of unprecedented size and quality. DEL represents an advance in medicinal chemistry which bridges the fields of… …   Wikipedia

  • Construct-Destruct — Studio album by Suicide Commando Released 1998 Genre …   Wikipedia

  • DNA vaccination — The making of a DNA vaccine. DNA vaccination is a technique for protecting an organism against disease by injecting it with genetically engineered DNA to produce an immunological response. Nucleic acid vaccines are still experimental, and have… …   Wikipedia

  • DNA — For a non technical introduction to the topic, see Introduction to genetics. For other uses, see DNA (disambiguation). The structure of the DNA double helix. The atoms in the structure are colour coded by element and the detailed structure of two …   Wikipedia

  • construct — An engineered chimeric DNA designed to be transferred into a cell or tissue. Typically, the construct comprises the gene or genes of interest, a marker gene and appropriate control sequences as a single package. A repeatedly used construct may be …   Glossary of Biotechnology

  • Recombinant DNA — (rDNA) molecules are DNA sequences that result from the use of laboratory methods (molecular cloning) to bring together genetic material from multiple sources, creating sequences that would not otherwise be found in biological organisms.… …   Wikipedia

  • United Kingdom National DNA Database — The United Kingdom National DNA Database (NDNAD; officially the UK National Criminal Intelligence DNA Database) is a national DNA Database that was set up in 1995. As of the end of 2005, it carried the profiles of around 3.1 million people. The… …   Wikipedia

  • Mitochondrial DNA — Human mitochondrial DNA …   Wikipedia

  • Gene knockout — A gene knockout (abbreviation: KO) is a genetic technique in which one of an organism s genes is made inoperative ( knocked out of the organism). Also known as knockout organisms or simply knockouts, they are used in learning about a gene that… …   Wikipedia

Share the article and excerpts

Direct link
… Do a right-click on the link above
and select “Copy Link”