ImageFile = Aminopterin.svg
IUPACName = ("2S")-2- [ [4- [(2,4-Diaminopteridin-6-yl)methylamino] benzoyl] amino] pentanedioic acid
OtherNames = 4-Aminofolic acid
Section1 = Chembox Identifiers
SMILES = C1=CC(=CC=C1C(=O)N [C@@H] (CCC(=O)O)C(=O)O)NCC2=CN=C3C(=N2)C(=NC(=N3)N)N
PubChem = 169371
ChemSpiderID = 2069
EINECS = 200-209-9
CASNo = 54-62-6
Section2 = Chembox Properties
Formula = C19H20N8O5
MolarMass= 440.413 g/mol
Aminopterin (4-aminopteroic acid), a 4-amino analog of
folic acid, is an antineoplasticdrug with immunosuppressiveproperties used in chemotherapy. Aminopterin is a synthetic derivative of pterin. Aminopterin works as an enzyme inhibitorby competing for the folate binding site of the enzyme dihydrofolate reductase. Its binding affinity for dihydrofolate reductase effectively blocks tetrahydrofolatesynthesis. This results in the depletion of nucleotideprecursors and inhibition of DNA, RNA, and protein synthesis.
antifolateactivity of aminopterin was first used by Sidney Farberin 1947 to induce remissions among children with leukemia. [cite web | url = http://www.dana-farber.org/abo/history/who/ | title = Who was Sidney Farber, MD? | accessdate = 2007-02-05 | publisher = Dana-Farber Cancer Institute] [cite journal | author = Farber S, Diamond LK, Mercer RD, Sylvester RF, Wolff JA | title = Temporary remissions in acute leukemia in children produced by folic acid antagonist, 4-Aminopteroyl-glutamic acid (Aminopterin) | year = 1948 | journal = N Engl J Med | volume = 238 | issue = 787 | pages = 787–93] Aminopterin was later marketed by Lederle Laboratories (Pearl River, New York) in the United States from 1953 to 1964 for the indication of pediatric leukemia. The closely related antifolate methotrexatewas simultaneously marketed by the company during the same period. Aminopterin was discontinued by Lederle Laboratories in favor of methotrexate due to manufacturing difficulties of the former.
During the period Aminopterin was marketed, the agent was used off-label to safely treat over 4,000 patients with psoriasis in the United States, producing dramatic clearing of lesions. [Rees, R. B., J. H. Bennett, et al. "Aminopterin for psoriasis: A decade's observation." The Archives of Dermatology. Volume 90, page 544, 1964.]
The use of aminopterin in cancer treatment was supplanted in the 1950s by
methotrexatedue to the latter's better therapeutic indexin a rodent tumor model. [Goldin, A., J. M. Venditti, et al. (1955). "A quantitative comparison of the antileukemic effectiveness of two folic acid antagonists in mice." J Natl Cancer Inst 15(6): 1657-1664.] Now in a more pure preparation and supported by laboratory evidence of superior tumor cell uptake "in vitro", aminopterin is being investigated in clinical trials in leukemia as a potentially superior antifolate to methotrexate.Citation | last = Cole | first = Peter D.| last2 = al.| first2 = et| title = Phase II Trial of Oral Aminopterin for Adults and Children with Refractory Acute Leukemia | journal = Clinical Cancer Research | volume = 11 | pages = 8089–8096 | date = November 15 2005| year = 2007 | url = http://clincancerres.aacrjournals.org/cgi/content/full/11/22/8089| doi = 10.1158/1078-0432.CCR-05-0355| pmid = 16299240 ]
The compound was explored as an
abortifacientin the 1960s and earlier, but was associated with congenital malformations. [cite journal | author = Emerson D | title = Congenital malformation due to attempted abortion with aminopterin | year = 1962 | journal = American Journal of Obstetrics and Gynecology | volume = 84 | issue = | pages = 356–357] Similar congenital abnormalities have been documented with methotrexate, and collectively their teratogeniceffects have become known as the "fetal aminopterin syndrome". When a similar cluster of abnormalies appears in the absence of exposure to antifolates it is referred to as aminopterin-like syndrome without aminopterin.cite web | url=http://www.nlm.nih.gov/mesh/jablonski/syndromes/syndrome289.html | title = Multiple Congenital Anomaly/Mental Retardation (MCA/MR) Syndromes: Fetal aminopterin syndrome | publisher= United States National Library of Medicine| accessdate = 2007-03-26 ]
Although the use of aminopterin as a
rodenticideis widely asserted on the web and elsewhere, there is no evidence that it has ever been used for that purpose either in the United States or elsewhere in the world.cite web
title= No Aminopterin in Tissues of Animals Killed by Recalled Pet Food
March 30, 2007
accessdate=2007-04-14] The preparation of the molecule is complex and expensive. It is also unstable in the environment due to degradation by light and heat. The apparently mistaken association of aminopterin with its use as a rodenticide likely dates back to a 1951 patent issued to the American Cyanamid Company (then the holding company of Lederle Laboratories) that is commonly cited by a variety of reference textbooks. [Alfred L. Franklin. United States Patent Number 2,575,168. "Rodenticide comprising 4-amino-pteroylglutamic acid." American Cyanamid Company, New York, NY.
November 13 1951.] Aminopterin has a single-dose LDLo of 2.5 mg/kg when orally administered to rats. [cite web | url = http://yosemite.epa.gov/oswer/ceppoehs.nsf/Profiles/54-62-6!OpenDocument | title = EPA Chemical Profile: Aminopterin | date = October 31, 1985| accessdate = 2007-03-23 | publisher = United States Environmental Protection Agency]
methotrexateare widely used in selection media (such as HAT medium) for cell culture.
Implication in 2007 Menu Foods recall
March 23 2007, ABC Newsreported [cite news
url = http://abcnews.go.com/US/story?id=2975912&page=1&US=true
title = Rat Poison to Blame for Pet Food Contamination
March 23 2007
accessdate = 2007-03-23
last = Kerley
first = David
ABC News] that aminopterin was the chemical linked to the 2007 Menu Foodspet food contamination incident. The incident resulted in a massive recall of the affected foods. [cite news
url = http://www.agmkt.state.ny.us/AD/alert.asp?ReleaseID=685
title = Menu Foods Issues Recall Of Specific Can And Small Foil Pouch Wet Pet Foods
March 16 2007
accessdate = 2007-03-25
publisher = New York Department of Agriculture] The link to aminopterin was confirmed by
New York StateAgriculture Commissioner Patrick Hooker and Dr. Donald Smith, Dean of Cornell University's College of Veterinary Medicine, in a statement released on the same day. [cite news
url = http://www.businessweek.com/ap/financialnews/D8O20JHO0.htm
title = Rat poison found in tainted pet food
March 23 2007
accessdate = 2007-03-23
last = Johnson
first = Mark
BusinessWeek] [cite news
url = http://www.agmkt.state.ny.us/AD/release.asp?ReleaseID=1598
title = New York Laboratories Identify Toxin In Recalled Pet Food
March 23 2007
accessdate = 2007-03-23
publisher = New York Department of Agriculture]
March 27, the ASPCAAnimal Poison Control Center expressed concern that the problem may not yet be fully understood and that other contaminants may be involved, noting that "clinical signs reported in cats affected by the contaminated foods are not fully consistent with the ingestion of rat poison containing aminopterin". [cite web
title=ASPCA Advises Caution As Pet Food Recall Crisis Grows; Other Contaminants May Be Involved in the Menu Foods Recall
March 27 2007
March 30it was widely reported that the United States Food and Drug Administrationhad found melaminein wheat gluten that was used in the pet foods in question. These same reports stated that the FDA had failed to find evidence of aminopterin in the wheat gluten. Tests at the University of Guelphin Ontario, Canada detected aminopterin in some pet food samples, but only in concentrations of parts per billionor parts per trillion, amounts too low to cause the symptoms seen. [cite news
url = http://www.cnn.com/2007/US/03/30/pet.food.recall.ap/index.html
title = FDA finds new chemical in recalled pet food, sick animals
March 30 2007
accessdate = 2007-03-30
Exposure and treatment
Symptoms of exposure in humans include:cite web
title=Chemical data sheet for Aminopterin
National Oceanic and Atmospheric Administration
work=CAMEO Chemicals] cite journal
title=The effect of delayed administration of folinic acid on immunological inhibition by methotrexate
month=March | year=1965
stomatitis– inflammation of the oral mucosa
pharyngitis– inflammation of the pharynx
*erythematous rashes – red rashes on the skin
hyperpigmentation– increased pigmentationassociated with cleared psoriatic lesions
renal failure– in high doses necessarily involving concomitant leucovorin rescue
Supralethal doses of aminopterin may be rescued with the antidote
leucovorin(also known as folinic acid), a reduced form of folic acidwhich bypasses dihydrofolate reductase, the enzyme inhibited by aminopterin. Leucovorin has been used in rats, dogs and humans to rescue aminopterin toxicity. [Nichol, C. A. and A. D. Welch (1950). "On the mechanism of action of aminopterin." "Proc Soc Exp Biol Med" 74: 403-411.] [ Rieselbach, R. E., E. E. Morse, et al. (1963). "Intrathecal aminopterin therapy of meningeal leukemia." "Arch Intern Med" 111: 620-30.] Glode, L. M., S. W. Pitman, et al. (1979). "A phase 1 study of high doses of aminopterin with leucovorin rescue in patients with advanced metastatic tumors." "Cancer Res" 39: 3707-3714.] Ratliff, A. F., J. Wilson, et al. (1998). "Phase I and pharmacokinetic trial of aminopterin in patients with refractory malignancies." "J Clin Oncol" 16(4): 1458-64.] Leucovorin rescue is a therapeutic maneuver intentionally employed with antifolates to achieve tumoricidal drug concentrations that would otherwise be lethal to the patient.
In humans, leucovorin rescue at overdosages lower than 10 mg aminopterin in an average 70 kg adult should comprise an initial leucovorin dose of at least 20 mg (10.0 mg/m2), given intravenously (preferably), or orally. Subsequent doses of 20 mg (which may be taken orally) should be given at 6 hour intervals until hematological abnormalities are improved.
Massive aminopterin overdosage in humans (i.e. > 40 mg AMT in an average 70 kg adult), should be approached with an initial leucovorin dose of 100 mg (50 mg/m2), given intravenously and continued at 6 hour intervals until the hematological abnormalities are improved (likely 8-12 courses or more). Additionally, to prevent reversible aminopterin-mediated nephrotoxicity manifesting as increases in serum creatinine and which further delays drug elimination, urinary alkalinization with NaHCO3 and volume expansion should be considered in cases of massive aminopterin overdosage, particularly those involving greater than 100 mg AMT in an average 70 kg adult human.
Consistent with the known enterohepatic cycling of the related antifolate methotrexate, oral
activated charcoal, and saline catharticor sorbitolmay promote excretion if an overdose of aminopterin is suspected. However, rescue with leucovorin should form the backbone of treatment.
The vitamin folic acid is an oxidized precursor to reduced folates that is upstream of the blockade at dihydrofolate reductase, and compared to leucovrin is recognized as a very weak antidote to the toxic effects of antifolates that is inappropriate for use in cases of acute intoxication. Minnich et al. dosed mongrel dogs subcutaneously with aminopterin and folic acid simultaneously to test whether folic acid can rescue animals from the lethality and toxicity of aminopterin [Minnich, V., C. V. Moore, et al. (1950). "Studies on the acute toxic effects of 4-amino-pteroylglutamic acid in dogs, guinea pigs and rabbits. Difference in species susceptibility and protective action of folic acid." "AMA Arch Pathol" 50(6): 787-99. ] Dogs were given 0.020, 0.046, 0.044 escalated to 0.088, and 0.097 mg/kg aminopterin each day for 7 to 12 days. Folic acid was given in a weight ratio to aminopterin of 200:1 to 800:1. All animals survived. In contrast, animals given aminopterin in an amount of 0.041 mg/kg/day x 6 days without folic acid died. Thus, when the ratio of folic acid to aminopterin was 200:1 and greater, all of the subjects survived on regimens that would have otherwise been uniformly fatal to all subjects.
Similar effects have been noted in rodent species as well, where the range for rescue by folic acid was fairly narrow and highly dependent on the timing (optimal of 1 hour prior to aminopterin) of administration in relation to aminopterin. [Franklin, A. L., E. L. R. Stokstad, et al. (1948). "Observations on the effect of 4-amino-pteroylglutamic acid in mice." Proc Soc Exp Biol Med 67: 398-400.] [Greenspan, E. M., A. Goldin, et al. (1950). "Studies on the mechanism of action of chemotherapeutic agents in cancer." "Cancer" 3: 856-863.] The temporal relationship between folic acid administration and rescue has been interpreted as the necessary period of time required for the vitamin to be converted "in vivo" to reduced forms.
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