Lipid peroxidation

Lipid peroxidation refers to the oxidative degradation of lipids. It is the process whereby free radicals "steal" electrons from the lipids in cell membranes, resulting in cell damage. This process proceeds by a free radical chain reaction mechanism. It most often affects polyunsaturated fatty acids, because they contain multiple double bonds in between which lie methylene -CH2- groups that possess especially reactive hydrogens. As with any radical reaction the reaction consists of three major steps: initiation, propagation and termination.

Initiation

Initiation is the step whereby a fatty acid radical is produced. The initiators in living cells are most notably reactive oxygen species (ROS), such as OH·, which combines with a hydrogen atom to make water and a fatty acid radical.

Propagation

The fatty acid radical is not a very stable molecule, so it reacts readily with molecular oxygen, thereby creating a peroxyl-fatty acid radical. This too is an unstable species that reacts with another free fatty acid producing a different fatty acid radical and a lipid peroxide or a cyclic peroxide if it had reacted with itself. This cycle continues as the new fatty acid radical reacts in the same way.

Termination

When a radical reacts it always produces another radical, which is why the process is called a "chain reaction mechanism." The radical reaction stops when two radicals react and produce a non-radical species. This happens only when the concentration of radical species is high enough for there to be a high probability of two radicals actually colliding. Living organisms have evolved different molecules that speed up termination by catching free radicals and therefore protect the cell membrane. One important such antioxidant is alpha-tocopherol, also known as vitamin E. Other anti-oxidants made within the body include the enzymes superoxide dismutase, catalase, and peroxidase.

Hazards

If not terminated fast enough, there will be damage to the cell membrane, which consists mainly of lipids. Phototherapy may cause hemolysis by rupturing red blood cell cell membranes in this way [ [http://www.blackwell-synergy.com/doi/abs/10.1111/j.1651-2227.1985.tb10987.x?journalCode=apa Red Cell Membrane Lipid Peroxidation and Hemolysis Secondary to Phototherapy] ENRIQUE M. OSTREA JR.11. Departments of Pediatrics, Wayne State University School of Medicine and Hutzel Hospital, Detroit, Michigan, USA]

In addition, end products of lipid peroxidation may be mutagenic and carcinogenic . For instance, the end product malondialdehyde reacts with deoxyadenosine and deoxyguanosine in DNA, forming DNA adducts to them, primarily M₁G.

Tests

Certain diagnostic tests are available for the quantification of the end products of lipid peroxidation, specifically malondialdehyde (MDA) [http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&list_uids=10064852&cmd=Retrieve&indexed=google Lipid peroxidation-DNA damage by malondialdehyde.] Marnett LJ. "Mutation research 1999 Mar 8;424(1-2):83-95". ] The most commonly used test is called a TBARS Assay.

References

External links

*