Cellular Microarray

Cellular Microarray

Cellular microarrays, are biological chips that allow interrogation of living cells on the surface of a solid support. The chips, spotted with varying materials, such as antibodies, proteins, or lipids, can interact with the cells, leading to their capture on specific spots. Combinations of different materials can be spotted in a given area, allowing not only cellular capture, when a specific interaction exists, but also the triggering of a cellular response, change in phenotype, or detection of a response from the cell, such as a specific secreted factor. An example of cellular microarrays are PMHC Cellular Microarrays.

Cellular microarrays were developed by Daniel Chen, Yoav Soen, Dan Kraft, Patrick Brown and Mark Davis at Stanford University Medical Center.

References

Chen DS, Davis MM (2006) Molecular and functional analysis using live cell microarrays. Curr Opin Chem Biol 10:28-34

Chen DS, Soen Y, Stuge TB, Lee PP, Weber JS, Brown PO, Davis MM (2005) Marked Differences in Human Melanoma Antigen-Specific T Cell Responsiveness after Vaccination Using a Functional Microarray. PLoS Med 2: 10: e265 (http://medicine.plosjournals.org/perlserv/?request=get-document&doi=10.1371%2Fjournal.pmed.0020265)

Soen Y., Chen D. S., Kraft D. L., Davis M. M. and Brown P.O. (2003) Detection and characterization of cellular immune responses using peptide-MHC microarrays. PLoS Biol. 1: E65 (http://biology.plosjournals.org/perlserv/?request=get-document&doi=10.1371/journal.pbio.0000065)

Chen DS, Davis MM (2005) Cellular immunotherapy: Antigen recognition is just the beginning. Springer Semin Immunopathol 27:119–127

Chen DS, Soen Y, Davis MM, Brown PO (2004) Functional and molecular profiling of heterogeneous tumor samples using a novel cellular microarray. J Clin Oncol 22:9507 (http://meeting.jco.org/cgi/content/abstract/22/14_suppl/9507)

Soen Y, Chen DS, Stuge TB, Weber JS, Lee PP, et al. (2004) A novel cellular microarray identifies functional deficiences in tumor-specific T cell responses. J Clin Oncol 22:2510


Wikimedia Foundation. 2010.

Игры ⚽ Поможем написать реферат

Look at other dictionaries:

  • Cellular neural network — Cellular neural networks (CNN) are a parallel computing paradigm similar to neural networks, with the difference that communication is allowed between neighbouring units only. Typical applications include image processing, analyzing 3D surfaces,… …   Wikipedia

  • Microarray — A microarray is a multiplex lab on a chip. It is a 2D array on a solid substrate (usually a glass slide or silicon thin film cell) that assays large amounts of biological material using high throughput screening methods. Types of microarrays… …   Wikipedia

  • Protein microarray — A protein microarray, sometimes referred to as a protein binding microarray, is a piece of glass on which different molecules of protein have been affixed at separate locations in an ordered manner thus forming a microscopic array. These are used …   Wikipedia

  • PMHC Cellular Microarrays — are a type of Cellular Microarray that has been spotted with pMHC complexes peptide MHC class I or peptide MHC class II.These biochips can be used to interrogate immune cells, particularly antigen specific T cells, from clinical samples for what… …   Wikipedia

  • Multiplex (assay) — For other uses of multiplex, see multiplex (disambiguation). A multiplex assay is a type of laboratory procedure that simultaneously measures multiple analytes (dozens or more) in a single assay. It is distinguished from procedures that measure… …   Wikipedia

  • MAGIChip — MAGIChips, also known as Microarrays of Gel immobilized Compounds on a chip or Three dimensional DNA Microarrays are devices for molecular hybridization produced by immobilizing oligonucleotides, DNA, enzymes, antibodies, and other compounds on a …   Wikipedia

  • Chemical biology — is a scientific discipline spanning the fields of chemistry and biology that involves the application of chemical techniques and tools, often compounds produced through synthetic chemistry, to the study and manipulation of biological systems.… …   Wikipedia

  • Gene expression profiling — Heat maps of gene expression values show how experimental conditions influenced production (expression) of mRNA for a set of genes. Green indicates reduced expression. Cluster analysis has placed a group of down regulated genes in the upper left… …   Wikipedia

  • Minimum Information Standards — Minimum Information (MI) standards or reporting guidelines specify the minimum amount of meta data (information) and data required to meet a specific aim or aims. Usually the aim is to provide enough meta data and data to enable the unambiguous… …   Wikipedia

  • Bioinformatics — For the journal, see Bioinformatics (journal). Map of the human X chromosome (from the NCBI website). Assembly of the human genome is one of the greatest achievements of bioinformatics. Bioinformatics …   Wikipedia

Share the article and excerpts

Direct link
… Do a right-click on the link above
and select “Copy Link”