Sequencing by hybridization
- Sequencing by hybridization
Sequencing by Hybridization is a class of methods for determining the order in which nucleotides occur on a strand of DNA. Typically used for looking for small changes relative to a known DNA sequence.The binding of one strand of DNA to its complementary strand in the DNA double-helix (aka hybridization) is sensitive to even single-base mismatches when the hybrid region is short or is specialized mismatch detection proteins are present. This is exploited in a variety of ways, most notable via DNA chips or microarrays with thousands to billions of synthetic oligonucleotides found in a genome of interest plus many known variations or even all possible single-base variations.
This technology has largely been displaced by Sequencing-by-synthesis based methods.
Examples of commercial systems
*Affymetrix
* [http://www.completegenomics.com/ Complete Genomics Inc.]
References
*Drmanac R et al. "Sequencing by hybridization (SBH): advantages, achievements, and opportunities." Adv Biochem Eng Biotechnol. 2002;77:75-101. PMID 12227738
*Hanna GJ et al. "Comparison of Sequencing by Hybridization and Cycle Sequencing for Genotyping of Human Immunodeficiency Virus Type 1 Reverse Transcriptase". J Clin Microbiol. 2000 July; 38(7): 2715–2721. PMID 10878069
*Church, George M. "Genomes for all." Scientific American. 2006 Jan;294(1):52. PMID 16468433
ee also
*Sequencing by ligation
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