- Protein nuclear magnetic resonance spectroscopy
Protein nuclear magnetic resonance spectroscopy (usually abbreviated protein NMR) is a field of
structural biologyin which NMR spectroscopyis used to obtain information about the structure and dynamics of proteins. The field was pioneered by, among others, Kurt Wüthrich, who shared the Nobel Prize in Chemistryin 2002. Protein NMR techniques are continually being used and improved in both academiaand the biotech industry. Structure determination by NMR spectroscopy usually consists of several following phases, each using a separate set of highly specialized techniques. The sample is prepared, resonances are assigned, restraints are generated and a structure is calculated and validated.
Protein nuclear magnetic resonance is performed on aqueous samples of highly purified protein. Usually the sample consist of between 300 and 600 microlitres with a protein concentration in the range 0.1 – 3 millimolar. The source of the protein can be either natural or produced in an expression system using
recombinant DNAtechniques through genetic engineering. Recombinantly expressed proteins are usually easier to produce in sufficient quantity, and makes isotopic labelling possible.
The most abundant isotopes of
carbonand oxygen, carbon-12and oxygen-16, have no net nuclear spin, which is the physical property nuclear magnetic resonance spectroscopy exploits. The most abundant isotope of nitrogen, nitrogen-14, has a net nuclear spin of 1; however, it also has a large quadrupolar moment, a property of the atomic nuclei which prevents high-resolution information to be obtained from this isotope. Thus nuclear magnetic resonance of proteins from natural sources is restricted to utilizing nuclear magnetic resonance based solely on protons. However the less common isotopes, carbon-13and nitrogen-15, have a net nuclear spin of 1/2, a simpler case making them suitable for nuclear magnetic resonance, and therefore labeling the proteins with these compounds opens up possibilities for doing more advanced experiments which also detect or use these nuclei. Isotopic labeling is done by growing the expression host in a growth mediaenriched with the desired isotopes. Since isotopically-enriched compounds remain expensive, organisms are used which are capable of growing on a defined minimal medium, containing only one carbon-13 source, usually glucose, but occasionally glycerolor methanol, and one nitrogen-15 source such as ammonium chlorideor ammonium sulfate. These organisms include organisms such as " Escherichia coli", which is the most frequently used type of bacteria, and " Pichia pastoris", which is the most frequently used yeast.
The purified protein is usually dissolved in a
buffer solutionand adjusted to the desired solvent conditions. The NMR sample is prepared in a thin walled glass tube.
Protein NMR utilizes multidimensional nuclear magnetic resonance experiments to obtain information about the protein. Ideally, each distinct nucleus in the molecule experiences a distinct chemical environment and thus has a distinct
chemical shiftby which it can be recognized. However, in large molecules such as proteins the number of resonances can typically be several thousand and a one-dimensional spectrum inevitably has incidental overlaps. Therefore multidimensional experiments are performed which correlate the frequencies of distinct nuclei. The additional dimensions decrease the chance of overlap and have a larger information content since they correlate signals from nuclei within a specific part of the molecule. Magnetization is transferred into the sample using pulses of electromagnetic ( radiofrequency) energy and between nuclei using delays; the process is described with so-called pulse sequences. Pulse sequences allow the experimenter to investigate and select specific types of connections between nuclei. The array of nuclear magnetic resonance experiments used on proteins fall in two main categories — one where magnetization is transferred through the chemical bonds, and one where the transfer is through space, irrespective of the bonding structure. The first category is used to assign the different chemical shifts to a specific nucleus, and the second is primarily used to generate the distance restraints used in the structure calculation, and in the assignment with unlabelled protein.
Depending on the concentration of the sample, on the magnetic field of the spectrometer, and on the type of experiment, a single multidimensional nuclear magnetic resonance experiment on a protein sample may take hours or even several days to obtain suitable signal-to-noise ratio through signal averaging, and to allow for sufficient evolution of magnetization transfer through the various dimensions of the experiment. Other things being equal, higher-dimensional experiments will take longer than lower-dimensional experiments.
Typically the first experiment to be measured with an isotope-labelled protein is a 2D
heteronuclear single quantum correlation(HSQC) spectrum where "heteronuclear" refers to nuclei other than 1H. In theory the heteronuclear single quantum correlation has one peak for each H bound to a heteronucleus. Thus in the 15N-HSQC one signal is expected for each amino acid residue with the exception of prolinewhich has no amide-hydrogen due to the cyclic nature of its backbone. Tryptophanand certain other residues with N-containing sidechains also give rise to additional signals. The 15N-HSQC is often referred to as the fingerprint of a protein because each protein has a unique pattern of signal positions. Analysis of the 15N-HSQC allows researchers to evaluate whether the expected number of peaks is present and thus to identify possible problems due to multiple conformations or sample heterogeneity. The relatively quick heteronuclear single quantum correlation experiment helps determine the feasibility of doing subsequent longer, more expensive, and more elaborate experiments. It is not possible to assign peaks to specific atoms from the heteronuclear single quantum correlation alone.
In order to analyze the nuclear magnetic resonance data, it is important to get a resonance assignment for the protein. That is to find out which
chemical shiftin each dimension corresponds to which atom. Several different types of experiments have been invented to achieve this. The procedure depends on whether the protein is isotopically labelled or not, since a lot of the assignment experiments depend on carbon-13 and nitrogen-15.
Homonuclear nuclear magnetic resonance
With unlabelled protein the usual procedure is to record a set of two dimensional homonuclear nuclear magnetic resonance experiments through
correlation spectroscopy(COSY), of which several types include conventional correlation spectroscopy, "total correlation" spectroscopy (TOCSY) and nuclear Overhauser effectspectroscopy (NOESY).ref|wuthrich A two-dimensional nuclear magnetic resonance experiment produces a two-dimensional spectrum. The units of both axes are chemical shifts. The COSY and TOCSY transfer magnetization through the chemical bonds between adjacent protons. The conventional correlation spectroscopy experiment is only able to transfer magnetization between protons on adjacent atoms, whereas in the total correlation spectroscopy experiment the protons are able to relay the magnetization, so it is transferred among all the protons that are connected by adjacent atoms. Thus in a conventional correlation spectroscopy, an alpha proton transfers magnetization to the beta protons, the beta protons transfers to the alpha and gamma protons, if any are present, then the gamma proton transfers to the beta and the delta protons, and the process continues. In total correlation spectroscopy, the alpha and all the other protons are able to transfer magnetization to the beta, gamma, delta, epsilon if they are connected by a continuous chain of protons. The continuous chain of protons are the sidechain of the individual amino acids. Thus these two experiments are used to build so called spin systems, that is build a list of resonances of the chemical shift of the peptide proton, the alpha protons and all the protons from each residue’s sidechain. Which chemical shifts corresponds to which nuclei in the spin system is determined by the conventional correlation spectroscopy connectivities and the fact that different types of protons have characteristic chemical shifts. To connect the different spinsystems in a sequential order, the nuclear Overhauser effect spectroscopy experiment has to be used. Because this experiment transfers magnetization through space, it will show crosspeaks for all protons that are close in space regardless of whether they are in the same spin system or not. The neighbouring residues are inherently close in space, so the assignments can be made by the peaks in the NOESY with other spin systems.
One important problem using homonuclear nuclear magnetic resonance is
overlapbetween peaks. This occurs when different protons have the same or very similar chemical shifts. This problem becomes greater as the protein becomes larger, so homonuclear nuclear magnetic resonance is usually restricted to small proteins or peptides.
Nitrogen-15 nuclear magnetic resonance
The process of resonance assignment for a nitrogen-15 labelled sample is similar to the homonuclear case. No experiment can be performed that transfers magnetisation between two spin systems through bonds either. The main difference is the ability to record nitrogen-15 edited three dimensional experiments: TOCSY-N HSQC and NOESY-N-HSQC. These experiments build onto the HSQC experiment, but have an additional proton dimension. It can be visualised as each peak in the HSQC having the TOCSY or NOESY peaks stacked onto it. Thus if the TOCSY peak from an amide proton, HN, has a cross peak to its alpha proton, Halpha, at the coordinates (HN, Halpha) in the TOCSY spectrum, the corresponding peak would be at (HN, Halpha,N) in the TOCSY-N-HSQC. Thus it is possible to resolve overlaps in the proton dimension, if the corresponding nitrogens have chemical shifts distinct from one another.
Carbon-13 and nitrogen-15 nuclear magnetic resonance
When the protein is labelled with carbon-13 and nitrogen-15 it is possible to record an experiment that transfers magnetisation over the peptide bond, and thus connect different spin systems through bonds. This is usually done using some of the following experiments, HNCO, HNCACO, HNCA,ref|hnca HNCOCA, HNCACB and CBCACONH. All six experiments consist of a HSQC plane expanded with a carbon dimension. In the HNCACO the spectrum contains peaks at the chemical shifts of the carbonyl carbons in the residue of the HSQC peak and the previous one in the sequence. The HNCO only contains the chemical shift from the previous residue, and it is thus possible to assign the carbonyl carbon shifts that corresponds to each HSQC peak and the one previous to that one. Sequential assignment can then be undertaken by matching the shifts of each spin system's own and previous carbons. The HNCA and HNCOCA works similarly, just with the alpha carbons rather than the carbonyls, and the HNCACB and the CBCACONH contains both the alpha carbon and the beta carbon. Usually several of these experiments are required to resolve overlap in the carbon dimension. This procedure is usually less ambiguous than the NOESY based method, since it is based on through bond transfer. In the NOESY-based methods additional peaks that are close in space but not belonging to the sequential residues will appear confusing the assignment process. When the sequential assignment has been made it is usually possible to assign the sidechains using HCCH-TOCSY, which is basically a TOCSY experiment resolved in an additional carbon dimension.
In order to make structure calculations a number of experimentially determined restraints have to be generated. These fall into different categories, the most widely used is distance restraints and angle restraints.
A crosspeak in a
NOESYexperiment signifies spatial proximity between the two nuclei in question. Thus each peak can be converted in to a maximum distance between the nuclei, usually between 1,8 and 6 angstroms. The intensity of a noesy peak is proportional to the distance to the minus 6th power, so the distance is determined according to intensity of the peak. The intensity-distance relationship is not exact, so usually a distance range is used.
It is of great importance to assign the noesy peaks to the correct nuclei based on the chemical shifts. If this task is performed manually it is usually very labor intensive, since proteins usually have thousands of noesy peaks. Some computer programs such as
CYANAref|cyana and ARIAref|aria2/CNS perform this task automatically, coupled to a structure calculation.
To obtain as accurate assignments as possible it is a great advantage to have access to carbon-13 and nitrogen-15 noesy experiments, since they help to resolve overlap in the proton dimension. This leads to faster and more reliable assignments, and in turn to better structures.
In addition to distance restraints, restraints on the torsion angles of the chemical bonds, typically the psi and phi angles can be generated. One approach is to use the
Karplus equation, to generate angle restraints from coupling constants. Another approach uses the chemical shifts to generate angle restraints. Both methods use the fact that the geometry around the alpha carbon affects the coupling constants and chemical shifts, so given the coupling constants or the chemical shifts, a qualified guess can be made about the torsion angles.
The analyte molecules in a sample can be partially ordered with respect to the external magnetic field of the spectrometer by manipulating the sample conditions. Common techniques include addition of
bacteriophages or bicelles to the sample, or preparation of the sample in a stretched polyacrylamide gel. This creates a local environment that favours certain orientations of nonspherical molecules. Normally in solution NMR the dipolar couplings between nuclei are averaged out because of the fast tumbling of the molecule. The slight overpopulation of one orientation means that a residual dipolar couplingremains to be observed. The dipolar coupling is commonly used in solid state NMR and provides information about the relative orientation of the bond vectors relative to a single global reference frame. Typically the orientation of the N-H vector is probed in a HSQC like experiment. Initially residual dipolar couplings were used for refinement of previously determined structures, but attempts at de novo structure determination have also been made.ref|rdc
NMR spectroscopy is nuclei specific. Thus it can distinguish between hydrogen and deuterium. The amide protons in the protein exchange readily with the solvent, and if the solvent contains a different isotope, typically
deuterium, the reaction can be monitored by NMR spectroscopy. How rapidly a given amide exchanges reflects its solvent accessibility. Thus amide exchange rates can give information on which parts of the protein are buried, hydrogen bonded etc. A common application is to compare the exchange of a free form versus a complex. The amides that become protected in the complex, are assumed to be in the interaction interface.
The experimentially determined restraints can be used as input for the structure calculation process. Researchers, using computer programs such as
CYANAor XPLOR-NIH,ref|xplor attempt to satisfy as many of the restraints as possible, in addition to general properties of proteins such as bond lengths and angles. The algorithms convert the restraints and the general protein properties into energy terms, and thus tries to minimize the energy. The process results in an ensemble of structures that, if the data were sufficient to dictate a certain fold, will converge.
In addition to structures,
nuclear magnetic resonancecan yield information on the dynamics of various parts of the protein. This usually involves measuring relaxation times such as T1 and T2 to determine order parameters, correlation times, and chemical exchange rates. NMR relaxation is a consequence of local fluctuating magnetic fieldswithin a molecule. Local fluctuating magnetic fields are generated by molecular motions. In this way measurements of relaxation times can provide information of motions within a molecule on the atomic level. In NMR studies of protein dynamics the nitrogen-15isotope is the preferred nucleus to study, because its relaxation times are relatively simple to relate to molecular motions, which however requires isotope labeling of the protein. The T1 and T2 relaxation times can be measured using various types of HSQCbased experiments. The types of motions, which can be detected, are motions that occur on a time-scale ranging from about 10 picoseconds to about 10 nanoseconds. In addition slower motions, which take place on a time-scale ranging from about 10 microseconds to 100 milliseconds can also be studied. However, since nitrogen atoms are mainly found in the backbone of a protein, the results mainly reflect the motions of the backbone, which is the most rigid part of a protein molecule. Thus, the results obtained from nitrogen-15relaxation measurements may not be representative for the whole protein. Therefore techniques utilizing relaxation measurements of carbon-13and deuteriumhave recently been developed, which enables systematic studies of motions of the amino acid side chains in proteins.
NMR spectroscopy on large proteins
Traditionally nuclear magnetic resonance spectroscopy has been limited to relatively small proteins or protein domains. This is in part caused by problems resolving overlapping peaks in larger proteins, but this has been alleviated by the introduction of isotope labelling and multidimensional experiments. Another more serious problem is the fact that in large proteins the magnetization relaxes faster, which means there is less time to detect the signal. This in turn causes the peaks to become broader and weaker, and eventually disappear. Two techniques have been introduced to attenuate the relaxation: transverse relaxation optimized spectroscopy (TROSY)ref|pervushin and deuterationref|markus of proteins. By using these techniques it has been possible to study proteins in complex with the 900 kDa chaperone GroES-GroEL.ref|fiaux
Automation of the process
Structure determination by NMR has traditionally been a time consuming process, requiring interactive analysis of the data by a trained scientist. There has been a considerable interest in automating the process to increase the throughput of structure determination (See
structural genomics). The two most time consuming processes are the resonance assignment and the NOE assignment. Several different computer programs have been published that do this processes automatically.ref|cyanaref|aria Efforts have also been made to standardize the structure calculation protocol to make it quicker and more amenable to automation.ref|liu
Nuclear magnetic resonance
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* Gordon S. Rule, T. Kevin Hitchens (2006). "Fundamentals of Protein NMR Spectroscopy". Springer. ISBN 1-4020-3499-7. http://www.springer.com/1-4020-3499-7
*Quincy Teng, (2005). "Structural Biology, Practical NMR Applications, Springer, ISBN 0-387-24367-4
*John Cavanagh, Wayne J. Fairbrother, Arthur G. Palmer III, Nicholas J. Skelton, (1995). "Protein NMR Spectroscopy: Principles and Practice". Academic Press. ISBN 0-12-164490-1.
* Kurt Wuthrich (1986) "NMR of Proteins and Nucleic Acids ". Wiley-Interscience. ISBN 0-471-82893-9
* [http://www.natureprotocols.com/2006/11/09/noesybased_strategy_for_assign.php NOESY-Based Strategy for Assignments of Backbone and Side Chain Resonances of Large Proteins without Deuteration (a protocol)]
* [https://prosa.services.came.sbg.ac.at/prosa.php ProSA-web] Web service for the recognition of errors in experimentally or theoretically determined protein structures
* [http://www.nmrwiki.org "NMRW"iki.ORG] NMR resource you can edit
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