West Virginia Route 527

Infobox road
state=WV
type=WV
route=527
length_mi=2.25
length_round=
length_ref=
established=
direction_a=South
starting_terminus=
junction=
direction_b=North
ending_terminus=
counties=Cabell
previous_type=US
previous_route=522
next_type=WV
next_route=598

West Virginia Route 527 is a north-south state highway located entirely in the Huntington, West Virginia area. The southern terminus of the route is at Interstate 64 exit 8 south of the city, where WV 527 becomes West Virginia Route 152 upon crossing the expressway. The northern terminus is on the West Virginia Department of Transportation-maintained Robert C. Byrd Bridge, better known as the Sixth Street Bridge, where the route becomes State Route 527 upon crossing into Ohio. In Ohio, SR 527 continues northward to State Route 7 in Chesapeake.

While the current Sixth Street Bridge was opened to traffic in 1995, its predecessor once carried U.S. Route 52 across the Ohio River to SR 7, hence the current numbering.

The southern terminus of WV 527 is most unusual, as it also serves as the northern terminus of WV 152, which continues along the same street as it passes over I-64 (both state routes are indicated on exit signage on the expressway). WV 152 continues along the former routing of US 52 until its intersection with US 52 four miles north of Crum.