Moeller stain

Endospores are surrounded be a highly resistant spore coat, The spore coat is highly resistant to excessive heat, freezing and desiccation as well as chemical agents. More importantly, for identification, spores are resistant to commonly employed staining techniques; therefore alternative staining methods are required. Moeller staining involves the use of a steamed dye reagent in order to increase the stainability of the spores; malachite green is the primary stain used in this experiment.

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  Endospore

Further reading

• Hayama, Masayoshi; Oana, Kozue; Kozakai, Tomonori; Umeda, Shuhei; Fujimoto, Junkoh; Ota, Hiroyoshi; Kawakami, Yoshiyuki (2007). "Proposal of a simplified technique for staining bacterial spores without applying heat - Successful modification of Moeller's method". European Journal of Medical Research 12 (8): 356–9. PMID 17933713. hdl:10091/10132.