Brefeldin A

Chembox new
ImageFile=Brefeldin_a.pngImageSize=
IUPACName=γ,4-Dihydroxy-2-(6-hydroxy-1-heptenyl)-4-cyclopentanecrotonic acid λ-lactone
OtherNames=
Section1= Chembox Identifiers
CASNo=20350-15-6
PubChem=24278285
SMILES=
MeSHName=
Section2= Chembox Properties
Formula=C₁₆H₂₄O₄
MolarMass=280.36
Appearance=
Density=
MeltingPt=
BoilingPt=
Solubility=
Section3= Chembox Hazards
MainHazards=
FlashPt=
Autoignition=

Brefeldin A is a lactone antibiotic produced by fungal organisms such as "Eupenicillium brefeldianum". Brefeldin A interferes with anterograde protein transport from the endoplasmic reticulum to the Golgi apparatus by inhibiting transport in Golgi, which leads to proteins accumulating inside the ER. In mammalian and yeast cells, the main target of brefeldin A appears to be a certain type of GTP-exchange factors responsible for activating a GTPase called Arf1p ; in turn, Arf1p is involved in the formation of transport vesicles by recruiting coat proteins to intracellular membranes. Brefeldin A was initially isolated as an anti-viral antibiotic [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=4299889&dopt=Citation] but is now primarily used in biological research to study protein transport.

Physical data

*Molecular formula: C₁₆H₂₄O₄
*Appearance: White to off-white crystalline powder
*Solubility: Clear colorless solution at 10mg/ml Dichloromethane.
*Clear colorless solution at 10mg/ml Methanol.
*Melting point: 204°C-205°C

Brefeldin A affects COPI coat formation, which is involved in retrograde transport. It inhibits ArfI activation, prohibiting recruitment of COPI coat proteins to the budding vessicle by binding to its guanine exchange factor.

External links

* [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=1740466&dopt=Citation Brefeldin A: insights into the control of membrane traffic and organelle structure.] (Review, 1992)
* [http://www.plantphysiol.org/cgi/content/full/130/3/1102 Brefeldin A: Deciphering an Enigmatic Inhibitor of Secretion.] (Review, 2002)
* [http://home.ncifcrf.gov/mtdp/Catalog/compounds/56310.html NCI Frederick, Structure and Data for Brefeldin A.] (Image)