Moving boundary electrophoresis
- Moving boundary electrophoresis
Moving boundary electrophoresis is electrophoresis in a free solution. It was developed by Arne Tiselius in 1937. Tiselius was awarded the 1948 Nobel Prize in chemistry for his work on the separation of colloids through electrophoresis, the motion of charged particles through a stationary liquid under the influence of an electric field.
The apparatus includes a U-shaped cell filled with buffer solution and electrodes immersed at its ends. The sample applied could be any mixture of charged components like a protein mixture. On applying voltage, the compounds will migrate to the anode or cathode depending on their charges. The change in the refractive index at the boundary of the separated compounds is detected using Schlieren optics at both ends of the solution in the cell. [Reiner, Westermeier , "Electrophoresis in Practice", 3rd Edition.]
References
External links
* [http://www.geocities.com/bioelectrochemistry/tiselius.htm Arne Wilhelm Kaurin Tiselius] — Information on Tiselius compiled from various sources.
Wikimedia Foundation.
2010.
Look at other dictionaries:
Moving-boundary electrophoresis — or free boundary electrophoresis is electrophoresis in a free solution. It was developed by Arne Tiselius in 1937. Tiselius was awarded the 1948 Nobel Prize in chemistry for his work on the separation of colloids through electrophoresis, the… … Wikipedia
moving boundary electrophoresis — the original method of electrophoresis, in which the movement of the solvent is unrestricted and all of the particles of a species move at the same rate, maintaining a sharp boundary which can be optically monitored … Medical dictionary
electrophoresis — electrophoretic /i lek troh feuh ret ik/, adj. /i lek troh feuh ree sis/, n. 1. Also called cataphoresis. Physical Chem. the motion of colloidal particles suspended in a fluid medium, due to the influence of an electric field on the medium. 2.… … Universalium
Harvey Itano — Harvey Akio Itano (born November 3, 1920) is an American biochemist best known for his work on the molecular basis of sickle cell anemia and other diseases. In collaboration with Linus Pauling, Itano used electrophoresis to demonstrate the… … Wikipedia
SDS-PAGE — PAGE redirects here. For other uses, see Page (disambiguation). Picture of an SDS PAGE. The molecular marker is in the left lane SDS PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a collection of related techniques… … Wikipedia
Arne Tiselius — Infobox Scientist name = Arne Wilhelm Kaurin Tiselius box width = image width = Arne Wilhelm Kaurin Tiselius caption = birth date = August 10, 1902 birth place = Stockholm, Sweden death date = death date and age|1971|10|29|1902|8|10 death place … Wikipedia
History of electrochemistry — Electrochemistry, a branch of chemistry, went through several changes during its evolution from early principles related to magnets in the early 16th and 17th centuries, to complex theories involving conductivity, electrical charge and… … Wikipedia
Arne Tiselius — Arne Tiselius, 1948 Arne Wilhelm Kaurin Tiselius (* 10. August 1902 in Stockholm; † 29. Oktober 1971 in Uppsala) war ein schwedischer Chemiker und Nobelpreisträger. Inhaltsverzeichnis … Deutsch Wikipedia
Glossary of fuel cell terms — The Glossary of fuel cell terms lists the definitions of many terms used within the fuel cell industry. The terms in this glossary may be used by fuel cell industry associations, in education material and fuel cell codes and standards to name but … Wikipedia
Mixture model — See also: Mixture distribution In statistics, a mixture model is a probabilistic model for representing the presence of sub populations within an overall population, without requiring that an observed data set should identify the sub population… … Wikipedia
