Multiple cloning site

Multiple cloning site

A multiple cloning site (MCS), also called a polylinker, is a short segment of DNA which contains many (up to ~20) restriction sites - a standard feature of engineered plasmids.[1] Restriction sites within an MCS are typically unique, occurring only once within a given plasmid. MCSs are commonly used during procedures involving molecular cloning or subcloning. Extremely useful in biotechnology, bioengineering, and molecular genetics, MCSs let a biotechnologist insert a piece of DNA or several pieces of DNA into the region of the MCS. This can be used to create transgenic organisms, also known as genetically modified organisms (GMOs).

The pUC18 and pUC19 polylinker

One bacterial plasmid used in genetic engineering as a plasmid cloning vector is pUC18. Its polylinker region is composed of several restriction enzyme recognition sites, that have been engineered into a single cluster (the polylinker). It has restriction sites for various restriction enzymes, including EcoRI, BamHI and PstI. Another vector used in genetic engineering is pUC19, which is similar to pUC18, but its polylinker region is reversed.

References

External links


Wikimedia Foundation. 2010.

Игры ⚽ Нужно решить контрольную?

Look at other dictionaries:

  • Multiple Cloning Site — Ein Polylinker oder eine Multiple Cloning Site (MCS) ist ein DNA Abschnitt in einem Vektor, dessen Sequenz direkt hintereinander verschiedene Schnittstellen für Restriktionsendonukleasen enthält. Diese ermöglicht ein flexibles Klonieren, da aus… …   Deutsch Wikipedia

  • multiple cloning site — See polycloning site …   Dictionary of molecular biology

  • multiple cloning site — Abbreviation: MCS See: polylinker …   Glossary of Biotechnology

  • Cloning vector — The pGEX 3x plasmid is a popular cloning vector. A cloning vector is a small piece of DNA into which a foreign DNA fragment can be inserted. The insertion of the fragment into the cloning vector is carried out by treating the vehicle and the… …   Wikipedia

  • polycloning site — (= multiple cloning site; MCS) Region of a phage or plasmid vector that has been engineered to contain a series of restriction sites that are usually unique within the entire vector. This makes it particularly easy to insert or excise (subclone)… …   Dictionary of molecular biology

  • Multiple displacement amplification — (MDA) is a non PCR based DNA amplification technique. This method can rapidly amplify minute amount of DNA samples to reasonable quantity for genomic analysis. The reaction starts by annealing random hexamer primers to the template and DNA… …   Wikipedia

  • Cloning — For the cloning of human beings, see Human cloning. For other uses, see Cloning (disambiguation). The sea anemone, Anthopleura elegantissima in process of cloning Cloning in biology is the process of producing similar populations of …   Wikipedia

  • Molecular cloning — refers to a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms.[1] The use of the word cloning refers to the fact that the method involves the …   Wikipedia

  • MCS — Multiple Chemical Sensitivity (Medical » Physiology) *** Multiple Cloning Site (Medical » Human Genome) ** Multiple Chemical Sensitivities (Medical » Human Genome) * Media Convergence Server (Computing » Networking) * Mesoscale Convective System… …   Abbreviations dictionary

  • Restriction enzyme — Glossary Restriction …   Wikipedia

Share the article and excerpts

Direct link
Do a right-click on the link above
and select “Copy Link”