BamHI

BamHI
restriction endonuclease bamhi bound to a non-specific dna.
Identifiers
Symbol	BamHI
Pfam	PF02923
Pfam clan	CL0236
InterPro	IPR004194
SCOP	1bhm
Available protein structures: Pfam structures PDB RCSB PDB; PDBe PDBsum structure summary

BamHI is a restriction enzyme, derived from Bacillus amyloliquefaciens. It has the recognition site (G'GATCC), and leaves a sticky end.^[1][2][3] One of the earlier enzymes to be used, it is popular for historical reasons, but also because digestion leaves a GATC overhang compatible with many other enzymes. Persistent issues with this specific enzyme spontaneously failing to generate cloneable fragments necessitate aliquoting prior to use. Engineered variants of the enzyme have been created in order to avoid star activity.

Recognition site

G G A T C C
C C T A G G

As at the end of 2010, there were 5 crystal structures of BamH1 in the Protein Data Bank

References

1. ^ Molecular cell biology. Lodish, Harvey F. 5. ed. : - New York : W. H. Freeman and Co., 2003, 973 s. b ill. ISBN 0-7167-4366-3
2. ^ Newman M, Strzelecka T, Dorner LF, Schildkraut I, Aggarwal AK (April 1994). "Structure of restriction endonuclease BamHI and its relationship to EcoRI". Nature 368 (6472): 660–4. doi:10.1038/368660a0. PMID 8145855. 
3. ^ Viadiu H, Aggarwal AK (May 2000). "Structure of BamHI bound to nonspecific DNA: a model for DNA sliding". Mol. Cell 5 (5): 889–95. doi:10.1016/S1097-2765(00)80329-9. PMID 10882125. 

External links

• MeSH Deoxyribonuclease+BamHI

This article includes text from the public domain Pfam and InterPro IPR004194