DGUOK

Deoxyguanosine kinase
PDB rendering based on 2ocp.
Available structures PDB 2ocp
Identifiers
Symbols	DGUOK; MTDPS3; dGK
External IDs	OMIM: 601465 MGI: 1351602 HomoloGene: 8456 GeneCards: DGUOK Gene
EC number	2.7.1.113
Gene Ontology Molecular function • nucleotide binding • deoxyguanosine kinase activity • ATP binding • kinase activity • transferase activity • phosphotransferase activity, alcohol group as acceptor • nucleoside kinase activity Cellular component • cytoplasm • mitochondrion • mitochondrial matrix • cytosol Biological process • purine base metabolic process • protein phosphorylation • guanosine metabolic process • nucleotide biosynthetic process • purine-containing compound salvage • dGTP metabolic process • purine deoxyribonucleoside metabolic process • nucleobase, nucleoside and nucleotide metabolic process Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	1716	27369
Ensembl	ENSG00000114956	ENSMUSG00000014554
UniProt	Q16854	Q504N4
RefSeq (mRNA)	NM_080916.1	NM_013764
RefSeq (protein)	NP_550438.1	NP_038792
Location (UCSC)	Chr 2: 74.15 – 74.19 Mb	Chr 6: 83.43 – 83.46 Mb
PubMed search	[1]	[2]

Deoxyguanosine kinase, mitochondrial is an enzyme that in humans is encoded by the DGUOK gene.^[1]

In mammalian cells, the phosphorylation of purine deoxyribonucleosides is mediated predominantly by two deoxyribonucleoside kinases, cytosolic deoxycytidine kinase and mitochondrial deoxyguanosine kinase. The protein encoded by this gene is responsible for phosphorylation of purine deoxyribonucleosides in the mitochondrial matrix. In addition, this protein phosphorylates several purine deoxyribonucleoside analogs used in the treatment of lymphoproliferative disorders, and this phosphorylation is critical for the effectiveness of the analogs. Alternative splice variants encoding different protein isoforms have been described for this gene.^[1]

References

1. ^ ^{a b} "Entrez Gene: DGUOK deoxyguanosine kinase". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=1716. 

External links

• GeneReview/NIH/UW entry on DGUOK-Related Mitochondrial DNA Depletion Syndrome, Hepatocerebral Form

Further reading

• Johansson M, Karlsson A (1996). "Cloning and expression of human deoxyguanosine kinase cDNA". Proc. Natl. Acad. Sci. U.S.A. 93 (14): 7258–62. doi:10.1073/pnas.93.14.7258. PMC 38970. PMID 8692979. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=38970. 
• Wang L, Hellman U, Eriksson S (1996). "Cloning and expression of human mitochondrial deoxyguanosine kinase cDNA". FEBS Lett. 390 (1): 39–43. doi:10.1016/0014-5793(96)00623-0. PMID 8706825. 
• Johansson M, Bajalica-Lagercrantz S, Lagercrantz J, Karlsson A (1997). "Localization of the human deoxyguanosine kinase gene (DGUOK) to chromosome 2p13". Genomics 38 (3): 450–1. doi:10.1006/geno.1996.0654. PMID 8975728. 
• Jüllig M, Eriksson S (2000). "Mitochondrial and submitochondrial localization of human deoxyguanosine kinase". Eur. J. Biochem. 267 (17): 5466–72. doi:10.1046/j.1432-1327.2000.01607.x. PMID 10951205. 
• Jüllig M, Eriksson S (2001). "Apoptosis induces efflux of the mitochondrial matrix enzyme deoxyguanosine kinase". J. Biol. Chem. 276 (26): 24000–4. doi:10.1074/jbc.M100435200. PMID 11294860. 
• Suzuki Y, Tsunoda T, Sese J et al. (2001). "Identification and Characterization of the Potential Promoter Regions of 1031 Kinds of Human Genes". Genome Res. 11 (5): 677–84. doi:10.1101/gr.164001. PMC 311086. PMID 11337467. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=311086. 
• Johansson K, Ramaswamy S, Ljungcrantz C et al. (2001). "Structural basis for substrate specificities of cellular deoxyribonucleoside kinases". Nat. Struct. Biol. 8 (7): 616–20. doi:10.1038/89661. PMID 11427893. 
• Mandel H, Szargel R, Labay V et al. (2001). "The deoxyguanosine kinase gene is mutated in individuals with depleted hepatocerebral mitochondrial DNA". Nat. Genet. 29 (3): 337–41. doi:10.1038/ng746. PMID 11687800. 
• Lotfi K, Månsson E, Peterson C et al. (2002). "Low level of mitochondrial deoxyguanosine kinase is the dominant factor in acquired resistance to 9-beta-D-arabinofuranosylguanine cytotoxicity". Biochem. Biophys. Res. Commun. 293 (5): 1489–96. doi:10.1016/S0006-291X(02)00415-1. PMID 12054684. 
• Salviati L, Sacconi S, Mancuso M et al. (2002). "Mitochondrial DNA depletion and dGK gene mutations". Ann. Neurol. 52 (3): 311–7. doi:10.1002/ana.10284. PMID 12205643. 
• Taanman JW, Kateeb I, Muntau AC et al. (2002). "A novel mutation in the deoxyguanosine kinase gene causing depletion of mitochondrial DNA". Ann. Neurol. 52 (2): 237–9. doi:10.1002/ana.10247. PMID 12210798. 
• Strausberg RL, Feingold EA, Grouse LH et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241. 
• Krawiec K, Kierdaszuk B, Shugar D (2003). "Inorganic tripolyphosphate (PPP(i)) as a phosphate donor for human deoxyribonucleoside kinases". Biochem. Biophys. Res. Commun. 301 (1): 192–7. doi:10.1016/S0006-291X(02)03007-3. PMID 12535661. 
• Gerhard DS, Wagner L, Feingold EA et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928. 
• Wang L, Limongelli A, Vila MR et al. (2005). "Molecular insight into mitochondrial DNA depletion syndrome in two patients with novel mutations in the deoxyguanosine kinase and thymidine kinase 2 genes". Mol. Genet. Metab. 84 (1): 75–82. doi:10.1016/j.ymgme.2004.09.005. PMID 15639197. 
• Mancuso M, Ferraris S, Pancrudo J et al. (2005). "New DGK gene mutations in the hepatocerebral form of mitochondrial DNA depletion syndrome". Arch. Neurol. 62 (5): 745–7. doi:10.1001/archneur.62.5.745. PMID 15883261. 
• Rual JF, Venkatesan K, Hao T et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514. 
• Fyrberg A, Mirzaee S, Lotfi K (2007). "Cell cycle dependent regulation of deoxycytidine kinase, deoxyguanosine kinase, and cytosolic 5'-nucleotidase I activity in MOLT-4 cells". Nucleosides Nucleotides Nucleic Acids 25 (9–11): 1201–4. doi:10.1080/15257770600894386. PMID 17065091. 
• Mousson de Camaret B (2007). "Kinetic properties of mutant deoxyguanosine kinase in a case of reversible hepatic mtDNA depletion". Biochem. J. 402 (2): 377–85. doi:10.1042/BJ20060705. PMC 1798436. PMID 17073823. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1798436. 
• Franco M, Johansson M, Karlsson A (2007). "Depletion of mitochondrial DNA by down-regulation of deoxyguanosine kinase expression in non-proliferating HeLa cells". Exp. Cell Res. 313 (12): 2687–94. doi:10.1016/j.yexcr.2007.04.003. PMID 17490647.