NUDT21

Nudix (nucleoside diphosphate linked moiety X)-type motif 21
PDB rendering based on 2cl3.
Available structures PDB 2CL3, 2J8Q, 3BAP, 3BHO, 3MDG, 3MDI, 3N9U, 3P5T, 3P6Y
Identifiers
Symbols	NUDT21; CFIM25; CPSF5; DKFZp686H1588
External IDs	OMIM: 604978 HomoloGene: 5090 GeneCards: NUDT21 Gene
Gene Ontology Molecular function • RNA binding • mRNA binding • protein binding • hydrolase activity • AU-rich element binding • protein homodimerization activity • histone deacetylase binding Cellular component • nucleus • nucleoplasm • centrosome • mRNA cleavage factor complex • paraspeckles Biological process • nuclear mRNA splicing, via spliceosome • transcription from RNA polymerase II promoter • termination of RNA polymerase II transcription • mRNA polyadenylation • mRNA processing • mRNA processing • RNA splicing • gene expression • mRNA 3'-end processing • protein tetramerization Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	11051	68219
Ensembl	ENSG00000167005	ENSMUSG00000031754
UniProt	O43809	n/a
RefSeq (mRNA)	NM_007006	NM_026623.3
RefSeq (protein)	NP_008937	NP_080899.1
Location (UCSC)	Chr 16: 56.46 – 56.49 Mb	Chr 8: 96.54 – 96.56 Mb
PubMed search	[1]	[2]

Cleavage and polyadenylation specificity factor subunit 5 is a protein that in humans is encoded by the NUDT21 gene.^[1][2]

The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. This gene encodes the 25kD subunit of the protein complex, which is composed of four polypeptides.^[2]

References

1. ^ Ruegsegger U, Blank D, Keller W (Jul 1998). "Human pre-mRNA cleavage factor Im is related to spliceosomal SR proteins and can be reconstituted in vitro from recombinant subunits". Mol Cell 1 (2): 243–53. doi:10.1016/S1097-2765(00)80025-8. PMID 9659921. 
2. ^ ^{a b} "Entrez Gene: NUDT21 nudix (nucleoside diphosphate linked moiety X)-type motif 21". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=11051. 

Further reading

• Rüegsegger U, Beyer K, Keller W (1996). "Purification and characterization of human cleavage factor Im involved in the 3' end processing of messenger RNA precursors". J. Biol. Chem. 271 (11): 6107–13. doi:10.1074/jbc.271.11.6107. PMID 8626397. 
• de Vries H, Rüegsegger U, Hübner W et al. (2000). "Human pre-mRNA cleavage factor IIm contains homologs of yeast proteins and bridges two other cleavage factors". EMBO J. 19 (21): 5895–904. doi:10.1093/emboj/19.21.5895. PMC 305781. PMID 11060040. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=305781. 
• Hartley JL, Temple GF, Brasch MA (2001). "DNA Cloning Using In Vitro Site-Specific Recombination". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=310948. 
• Wiemann S, Weil B, Wellenreuther R et al. (2001). "Toward a Catalog of Human Genes and Proteins: Sequencing and Analysis of 500 Novel Complete Protein Coding Human cDNAs". Genome Res. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=311072. 
• Simpson JC, Wellenreuther R, Poustka A et al. (2001). "Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing". EMBO Rep. 1 (3): 287–92. doi:10.1093/embo-reports/kvd058. PMC 1083732. PMID 11256614. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1083732. 
• Kim H, Lee Y (2002). "Interaction of poly(A) polymerase with the 25-kDa subunit of cleavage factor I". Biochem. Biophys. Res. Commun. 289 (2): 513–8. doi:10.1006/bbrc.2001.5992. PMID 11716503. 
• Strausberg RL, Feingold EA, Grouse LH et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241. 
• Awasthi S, Alwine JC (2003). "Association of polyadenylation cleavage factor I with U1 snRNP". RNA 9 (11): 1400–9. doi:10.1261/rna.5104603. PMC 1287061. PMID 14561889. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1287061. 
• Brown KM, Gilmartin GM (2004). "A mechanism for the regulation of pre-mRNA 3' processing by human cleavage factor Im". Mol. Cell 12 (6): 1467–76. doi:10.1016/S1097-2765(03)00453-2. PMID 14690600. 
• Bouwmeester T, Bauch A, Ruffner H et al. (2004). "A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway". Nat. Cell Biol. 6 (2): 97–105. doi:10.1038/ncb1086. PMID 14743216. 
• Dettwiler S, Aringhieri C, Cardinale S et al. (2005). "Distinct sequence motifs within the 68-kDa subunit of cleavage factor Im mediate RNA binding, protein-protein interactions, and subcellular localization". J. Biol. Chem. 279 (34): 35788–97. doi:10.1074/jbc.M403927200. PMID 15169763. 
• Lehner B, Sanderson CM (2004). "A Protein Interaction Framework for Human mRNA Degradation". Genome Res. 14 (7): 1315–23. doi:10.1101/gr.2122004. PMC 442147. PMID 15231747. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=442147. 
• Gerhard DS, Wagner L, Feingold EA et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928. 
• Wiemann S, Arlt D, Huber W et al. (2004). "From ORFeome to Biology: A Functional Genomics Pipeline". Genome Res. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC 528930. PMID 15489336. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528930. 
• Rush J, Moritz A, Lee KA et al. (2005). "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells". Nat. Biotechnol. 23 (1): 94–101. doi:10.1038/nbt1046. PMID 15592455. 
• Andersen JS, Lam YW, Leung AK et al. (2005). "Nucleolar proteome dynamics". Nature 433 (7021): 77–83. doi:10.1038/nature03207. PMID 15635413. 
• Stelzl U, Worm U, Lalowski M et al. (2005). "A human protein-protein interaction network: a resource for annotating the proteome". Cell 122 (6): 957–68. doi:10.1016/j.cell.2005.08.029. PMID 16169070. 
• Rual JF, Venkatesan K, Hao T et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514. 
• Mehrle A, Rosenfelder H, Schupp I et al. (2006). "The LIFEdb database in 2006". Nucleic Acids Res. 34 (Database issue): D415–8. doi:10.1093/nar/gkj139. PMC 1347501. PMID 16381901. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1347501.