Pullulanase

Pullulanase is a specific kind of glucanase, an amylolytic exoenzyme, that degrades pullulan. It is produced as an extracellular, cell surface-anchored lipoprotein by Gram-negative bacteria of the genus Klebsiella. Type I pullulanases specifically attack α-1,6 linkages, while type II pullulanases are also able to hydrolyse α-1,4 linkages. It is also produced by some other bacteria and archaea. Pullulanase is used as a detergent in biotechnology.

Pullulanase (EC number|3.2.1.41) is also known as pullulan-6-glucanohydrolase (Debranching enzyme). Its substrate, pullulan, is regarded as a chain of maltotriose units linked by alpha-1,6-glycosidic bonds. Pullulanase will hydrolytically cleave pullulan (alpha-glucan polysaccharides).

External links

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* {Enhanced Production of Thermostable Pullulanase Type 1 Using Bacillus cereus FDTA 13 and Its Mutant; Subhash U. Nair, Rekha S. Singhal and Madhusudan Y. Kamat| http://tiaktiv.org/ftbrfd/44-275.pdf }
* {Screening of Microbial strains for pullulanase; Subhash U. Nair, Rekha S. Singhal and Madhusudan Y. Kamat; Biotechnological Approaches for Sustainable Development By M. Sudhakara Reddy;
* {Induction of pullulanase production in Bacillus cereus FDTA-13 [An article from: Bioresource Technology] ; Subhash U. Nair, Rekha S. Singhal and Madhusudan Y. Kamat