Methylaspartate ammonia-lyase

Methylaspartate ammonia-lyase
methylaspartate ammonia-lyase
Identifiers
EC number 4.3.1.2
CAS number 9033-26-5
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum
Gene Ontology AmiGO / EGO

In enzymology, a methylaspartate ammonia-lyase (EC 4.3.1.2) is an enzyme that catalyzes the chemical reaction

L-threo-3-methylaspartate \rightleftharpoons mesaconate + NH3

Hence, this enzyme has one substrate, L-threo-3-methylaspartate, and two products, mesaconate and NH3.

This enzyme belongs to the family of lyases, specifically ammonia lyases, which cleave carbon-nitrogen bonds. The systematic name of this enzyme class is L-threo-3-methylaspartate ammonia-lyase (mesaconate-forming). Other names in common use include beta-methylaspartase, 3-methylaspartase, and L-threo-3-methylaspartate ammonia-lyase. This enzyme participates in c5-branched dibasic acid metabolism and nitrogen metabolism. It employs one cofactor, cobamide.

Structural studies

As of late 2007, 4 structures have been solved for this class of enzymes, with PDB accession codes 1KCZ, 1KD0, 1KKO, and 1KKR.

References

  • BARKER HA, SMYTH RD, WAWSZKIEWICZ EJ, LEE MN, WILSON RM (1958). "Enzymic preparation and characterization of an alpha-L-beta-methylaspartic acid". Arch. Biochem. Biophys. 78 (2): 468–76. doi:10.1016/0003-9861(58)90371-0. PMID 13618029. 
  • Bright HJ and Ingraham LL (1960). "The preparation of crystalline beta-methylaspartase". Biochim. Biophys. Acta 44: 586–588. doi:10.1016/0006-3002(60)91612-7. 
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