- Opine dehydrogenase
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opine dehydrogenase Identifiers EC number 1.5.1.28 CAS number 108281-02-3 Databases IntEnz IntEnz view BRENDA BRENDA entry ExPASy NiceZyme view KEGG KEGG entry MetaCyc metabolic pathway PRIAM profile PDB structures RCSB PDB PDBe PDBsum Gene Ontology AmiGO / EGO Search PMC articles PubMed articles In enzymology, an opine dehydrogenase (EC 1.5.1.28) is an enzyme that catalyzes the chemical reaction
- (2S)-2-{[1-(R)-carboxyethyl]amino}pentanoate + NAD+ + H2O
L-2-aminopentanoic acid + pyruvate + NADH + H+
The 3 substrates of this enzyme are [[(2S)-2-{[1-(R)-carboxyethyl]amino}pentanoate]], NAD+, and H2O, whereas its 4 products are L-2-aminopentanoic acid, pyruvate, NADH, and H+.
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-NH group of donors with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is (2S)-2-{[1-(R)-carboxyethyl]amino}pentanoate:NAD+ oxidoreductase (L-aminopentanoate-forming). Other names in common use include (2S)-2-{[1-(R)-carboxyethyl]amino}pentanoate dehydrogenase (NAD+,, and L-aminopentanoate-forming).
References
- Asano Y, Yamaguchi K, Kondo K (1989). "A new NAD+-dependent opine dehydrogenase from Arthrobacter sp strain 1C". J. Bacteriol. 171 (8): 4466–71. PMC 210226. PMID 2753861. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=210226.
- Dairi T, Asano Y (1995). "Cloning, nucleotide sequencing, and expression of an opine dehydrogenase gene from Arthrobacter sp. strain 1C". Appl. Environ. Microbiol. 61 (8): 3169–71. PMC 167592. PMID 7487048. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=167592.
- Kato Y, Yamada H and Asano Y (1996). "Stereoselective synthesis of opine-type secondary amine carboxylic acids by a new enzyme opine dehydrogenase. Use of recombinant enzymes". J. Mol. Catal., B Enzym. 1 (3–6): 151–160. doi:10.1016/1381-1177(95)00011-9.
This EC 1.5 enzyme-related article is a stub. You can help Wikipedia by expanding it. - (2S)-2-{[1-(R)-carboxyethyl]amino}pentanoate + NAD+ + H2O
