Microelectrophoresis is a method of studying electrophoresis of various dispersed particles using optical microscopy. This method provides image of moving particles, which is its unique advantage.

Complexity of this method is associated with electro-osmosis generated by electric field influence on the double layers of the sample cell walls. In the usually used closed cells this creates Poiseuille type back flow, leading to parabolic velocity profile. There are two stationary layers, where fluid does not move. Position of these stationary layer regarding cell walls depends on the cell geometry. It is possible to focus microscope on this stationary layers and observe particles motion not affected with electro-osmosis.

The second complexity comes from necessity of diluting sample, if it was initially concentrated. Concentration must be sufficiently low for observing individual particles.

Measurement in the low polar fluids present additional problems. These systems have low electrical conductivity and low dielectric permittivity. Electrophoretic mobility is low and this requires high electric field, by factor 10 and higher.

Details of this method are presented in IUPAC Technical Report [1] prepared by a group of most known world experts on the electrokinetic phenomena.


  1. ^ ”Measurement and Interpretation of Electrokinetic Phenomena”, International Union of Pure and Applied Chemistry, Technical Report, published in Pure Appl.Chem., vol 77, 10, pp.1753-1805, 2005

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  • microelectrophoresis — noun Etymology: New Latin Date: 1930 electrophoresis in which the movement of single particles is observed in a microscope; also electrophoresis in which micromethods are used • microelectrophoretic adjective • microelectrophoretically adverb …   New Collegiate Dictionary

  • microelectrophoresis — microelectrophoretic /muy kroh i lek troh feuh ret ik/, adj. /muy kroh i lek troh feuh ree sis/, n. Chem. any of several techniques for observing, by means of a microscope or an ultramicroscope, the electrophoresis of minute surface particles.… …   Universalium

  • microelectrophoresis — noun The direct microscopic observation and measurement of the movement of particles during electrophoresis …   Wiktionary

  • microelectrophoresis — mi·cro·elec·tro·pho·re·sis .lek trə fə rē səs n, pl re·ses .sēz electrophoresis in which the movement of single particles is observed in a microscope also electrophoresis in which micromethods are used mi·cro·elec·tro·pho·ret·ic ret ik adj… …   Medical dictionary

  • microelectrophoresis — n. technique for observing the movement of microscopic particles in a fluid under the influence of a magnetic field (Chemistry) maɪkrəʊɪlektrÉ™fÉ™ rɪːsɪs …   English contemporary dictionary

  • microelectrophoresis — mi·cro·electrophoresis …   English syllables

  • microelectrophoresis — mi•cro•e•lec•tro•pho•re•sis [[t]ˌmaɪ kroʊ ɪˌlɛk troʊ fəˈri sɪs[/t]] n. chem. the observation and measurement of electrophoresis by a microscope • Etymology: 1955–60 mi cro•e•lec tro•pho•ret′ic ˈrɛt ɪk adj …   From formal English to slang

  • microelectrophoresis — …   Useful english dictionary

  • microelectrophoretic — adjective see microelectrophoresis …   New Collegiate Dictionary

  • microelectrophoretically — adverb see microelectrophoresis …   New Collegiate Dictionary

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