Santa Ana de Pusa

Infobox City
official_name = Santa Ana de Pusa, Spain | nickname = |

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| map_caption = | subdivision_type = Country | subdivision_name = Spain | subdivision_type1 = Autonomous community | subdivision_name1 = Castile-La Mancha | subdivision_type2 = Province | subdivision_name2 = Toledo | subdivision_type3 = Municipality | subdivision_name3 = Santa Ana de Pusa | leader_title = | leader_name = | area_magnitude = | area_total_km2 = 19 | area_land_km2 = | area_water_km2 = | elevation_m = 594 | population_as_of = 2006 | population_note = | population_total = 411 | population_density_km2 = 21.6 | timezone = CET | utc_offset = +1 | timezone_DST = CEST | utc_offset_DST = +2 | latitude = | longitude = | website = | footnotes = |

Santa Ana de Pusa is a municipality located in the province of Toledo, Castile-La Mancha, Spain. According to the 2006 census (INE), the municipality has a population of 411 inhabitants.