- Intramembranous ossification
Intramembranous ossification is one of the two processes during
fetal development of the mammalian skeletal system in whichbone tissue is created. It is also an essential process during the healing of bone fractures Brighton, Carl T. and Robert M. Hunt (1991), "Early histological and ultrastructural changes in medullary fracture callus", "Journal of Bone and Joint Surgery", 73-A (6): 832-847] and the rudimentary formation of bones of the head. Netter, Frank H. (1987), "Musculoskeletal system: anatomy, physiology, and metabolic disorders". Summit, New Jersey: Ciba-Geigy Corporation ISBN 0914168886, p.129] Unlikeendochondral ossification , which is the other process,cartilage is not present during intramembranous ossification.Creation of bone tissue
Mesenchymal stem cells, or MSCs, within human
mesenchyme or themedullary cavity of a bone fracture initiate the process of intramembranous ossification. An MSC is an unspecialized cell whose morphology undergoes characteristic changes as it develops into anosteoblast . MSCs are long and thin, and their smallcell body has a few cell processes that are also long and thin. The cell body contains a large, round nucleus with a prominentnucleolus which is surrounded by finely dispersedchromatin particles, giving the nucleus a clear appearance. The remainder of the cell body contains a small amount ofGolgi apparatus ,rough endoplasmic reticulum ,mitochondria , andpolyribosomes . The cells are widely dispersed and the adjacentextracellular matrix is populated by a few reticular fibrils but is devoid of the other types ofcollagen fibrils. This morphology is characteristic of a mesenchymal stem cell.Brighton and Hunt (1991)]Some of the MSCs begin to
replicate until they have formed a small, dense aggregation of cells, a nodule. At this point the MSCs stop replicating and changes in their morphology begin to occur. The cell body becomes larger and rounder, and the long, thin cell processes are no longer present. The amount of Golgi apparatus and rough endoplasmic reticulum increases. Eventually, the cells within the aggregate acquire the morphologic characteristics of an osteoprogenitor cell.Brighton and Hunt (1991)]Changes in the morphology of the osteoprogenitor cells begin to occur. Their shape becomes more columnar and the amount of Golgi apparatus and rough endoplasmic reticulum increases. The cells begin to create an extracellular matrix consisting of
Type-I collagen fibrils. This matrix isosteoid and the cells that created it areosteoblasts . The osteoblasts, while lining the periphery of the nodule, continue to create osteoid at its center.Brighton and Hunt (1991)]As the amount of osteoid increases some of the osteoblasts become incorpoated within it to become osteocytes. Eventually, the osteoid mineralizes. This small structure, which began as a diffuse collection of MSCs, contains osteoid which has become mineralized, is lined externally by active osteoblasts, and contains osteocytes. This small structure has become rudimentary bone tissue and this is a brief description of how it was created.Brighton and Hunt (1991)]
Overview
The first step in the process is the formation of bone spicules which eventually fuse with each other and become
trabeculae . Theperiosteum is formed and bone growth continues at the surface of trabeculae. Much like spicules, the increasing growth of trabeculae result in interconnection and this network is called woven bone. Eventually, woven bone is replaced by lamellar bone.Process Overview
*Mesenchyme cell in the membrane become osteochondral progenitor cell
*osteochondral progenitor cell specialized to become osteoblast
*Osteoblast produce bone matrix and surrounded collagen fiber and becomeosteocyte
*As the result process trabeculae will develop
*Osteoblast will trap trabeculae to produce bone
*Trabeculae will join together to produce spongy cell
*Cells in the spongy cell will specialize to produce red bone marrow
*Cells surrounding the developing bone will produce periosteum
*Osteoblasts from thePeriosteum on the bone matrix will produce compact boneFormation of bone spicules
Embryologic
mesenchymal cell s (MSC) condense into layers of vascularized primitiveconnective tissue . Certain mesenchymal cells group together, usually near or around blood vessels, and differentiate into osteogenic cells which deposit bone matrix constitutively. These aggregates of bony matrix are called bone spicules. Separate mesenchymal cells differentiate intoosteoblast s, which line up along the surface of the spicule and secrete moreosteoid , which increases the size of the spicule.Formation of woven bone
As the spicules continue to grow, they fuse with adjacent spicules and this results in the formation of trabeculae. When
osteoblast s become trapped in the matrix they secrete, they differentiate intoosteocyte s.Osteoblast s continue to line up on the surface which increases the size. As growth continues, trabeculae become interconnected and woven bone is formed. The term primary spongiosa is also used to refer to the initial trabecular network.Primary center of ossification
The
periosteum is formed around the trabeculae by differentiating mesenchymal cells. The primary center of ossification is the area where bone growth occurs between theperiosteum and the bone. Osteogenic cells that originate from theperiosteum increase appositional growth and a bone collar is formed. The bone collar is eventually mineralized and lamellar bone is formed.Formation of osteon
Osteon s are units or principal structures of compact bone. During the formation of bone spicules, cytoplasmic processes fromosteoblast s interconnect. This becomes the canaliculi ofosteon s. Since bone spicules tend to form aroundblood vessel s, the perivascular space is greatly reduced as the bone continues to grow. When replacement to compact bone occurs, this blood vessel becomes the central canal of the osteon.References
*Martin, RB; DB Burr; NA Sharkey (1998), "Skeletal Tissue Mechanics", Chapter 2, Springer-Verlag
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