RncO

The bacterial regulatory element (rncO) is found in the rnc leader sequence. The rnc operon is negatively auto-regulated by transcript stability. rnc, the first gene in the operon codes for RNase III which cleaves the long rncO stem II leading to transcript degradation and a reduction in translation.cite journal | author = Matsunaga J, Simons EL, Simons RW | title = RNase III autoregulation: structure and function of rncO, the posttranscriptional "operator" | journal = RNA | volume = 2 | issue = 12 | pages = 1228–40 | year = 1996 | pmid = 8972772 | doi = | issn = ] Matsunaga "et al". showed that RNase III cleavage can initiate rnc transcript decay independently of rnc gene translation.cite journal | author = Matsunaga J, Simons EL, Simons RW | title = Escherichia coli RNase III (rnc) autoregulation occurs independently of rnc gene translation | journal = Mol. Microbiol. | volume = 26 | issue = 5 | pages = 1125–35 | year = 1997 | pmid = 9426147 | doi = 10.1046/j.1365-2958.1997.6652007.x| issn = ]

tructure

Functionally the first 215 nucleotides of the rnc leader have been shown to be sufficient. Within this region three stem-loops were identified. Stem-loop II is cleaved by RNase III, whereas stem-loops I and III are important for stability.

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