PKC alpha

Protein kinase C, alpha, also known as PRKCA, is a human gene. PBB_Summary
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summary_text = Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca²⁺ handling in myocytes. [cite web | title = Entrez Gene: PRKCA protein kinase C, alpha| url = http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=5578| accessdate = ]

Background

Protein kinase C-alpha (PKC-α) is a specific member of the protein kinase family. These enzymes are characterized by their ability to add a phosphate group to other proteins, thus changing their function. PKC-α has been widely studied in the tissues of many organisms including drosophila, xenopus, cow, dog, chicken, human, monkey, mouse, pig, and rabbit. Many studies are currently being conducted investigating the structure, function, and regulation of this enzyme. The most recent investigations concerning this enzyme include its general regulation, hepatic function, and cardiac function.

Regulation

PKC-α is unique in its mode of regulation compared to other kinases within this family. In general, the protein kinase family is regulated by allosteric regulation, the binding of a modulating molecule that effects a conformational change in the enzyme and thus a change in the enzyme’s activity. The primary mode of PKC-α’s regulation, however, involves its interaction with the cell membrane, not direct interaction with specific molecules. [http://www.biophysj.org/cgi/reprint/76/2/916.pdf. Vicente Micol. Correlation between Protein Kinase C an Activity and MembranePhase Behavior. Departamento de Bioquı´mica y Biologı´a Molecular] The cell membrane consists of phospholipids. At warmer temperatures, phospholipids exist in a more fluid state as a result of increased intramolecular motion. The more fluid the cell membrane, the greater PKC-α’s activity. At cooler temperatures, phospholipids are found in a solid state with constricted motion. As phospholipids become stationary, they assume a particular orientation within the membrane. Phospholipids that solidify at an irregular or angled orientation with respect to the membrane, can reduce PKC-α’s activity. [http://www.biophysj.org/cgi/reprint/76/2/916.pdf. Vicente Micol. Correlation between Protein Kinase C an Activity and MembranePhase Behavior. Departamento de Bioquı´mica y Biologı´a Molecular]

The composition of the cell membrane can also affect PKC-α’s function. The presence of calcium ions, magnesium ions, and diacylglycerols (DAGs) are the most important because they influence the hydrophobic domain of the membrane. Varying concentrations of these three components constitute a longer or shorter length of the hydrophobic domain. Membranes with long hydrophobic domains result in decreased activity because it is harder for PKC-α to insert into the membrane. At low concentrations, the hydrophobic domain is shorter allowing PKC-α to readily insert into the membrane and its activity increases. [http://www.biophysj.org/cgi/reprint/76/2/916.pdf. Vicente Micol. Correlation between Protein Kinase C an Activity and MembranePhase Behavior. Departamento de Bioquı´mica y Biologı´a Molecular]

econdary Protein Structure Determination

Using infrared spectroscopy techniques, researchers have demonstrated that the secondary structure of PKC alpha consists of around 44% beta sheets and nearly 22% alpha helices at 20°C. [http://pubs.acs.org/cgi-bin/article.cgi/bichaw/2004/43/i08/html/bi035128i.html An Infrared Spectroscopic Study of the Secondary Structure of Protein Kinase C and Its Thermal Denaturation

Alejandro Torrecillas, Senena Corbalán-García, and Juan C. Gómez-Fernández*

Departamento de Bioquímica y Biología Molecular (A), Facultad de Veterinaria, Universidad de Murcia. Apartado de Correos 4021, E-30080-Murcia, Spain ] Upon addition of calcium ions, a slight increase in beta sheets to 48% was observed. Additional ligands normally associated with PKC alpha, such as PMA, ATP, and phospholipids had no effect on secondary structure. [http://pubs.acs.org/cgi-bin/article.cgi/bichaw/2004/43/i08/html/bi035128i.html An Infrared Spectroscopic Study of the Secondary Structure of Protein Kinase C and Its Thermal Denaturation

Alejandro Torrecillas, Senena Corbalán-García, and Juan C. Gómez-Fernández*

Departamento de Bioquímica y Biología Molecular (A), Facultad de Veterinaria, Universidad de Murcia. Apartado de Correos 4021, E-30080-Murcia, Spain ]

Interestingly, the structure of PKC alpha was better preserved during denaturation of the enzyme at 75°C in the presence of calcium ions than in their absence. In one study, beta sheet composition only decreased by 13% with calcium ions present compared to 19% when absent. [http://pubs.acs.org/cgi-bin/article.cgi/bichaw/2004/43/i08/html/bi035128i.html An Infrared Spectroscopic Study of the Secondary Structure of Protein Kinase C and Its Thermal Denaturation

Alejandro Torrecillas, Senena Corbalán-García, and Juan C. Gómez-Fernández*

Departamento de Bioquímica y Biología Molecular (A), Facultad de Veterinaria, Universidad de Murcia. Apartado de Correos 4021, E-30080-Murcia, Spain ]

Current Research Involving Protein Kinase C alpha

Epithelial Studies

Another field of research has indicated that PKC-α plays a vital role in epithelial tissue, the tissue that covers all external and internal surfaces of the body. Specifically, PKC-α is involved in altering the function of tight junctions. Tight junctions exist at the meeting point between two cells. Here, tight junctions fuse together to form an impermeable barrier to not only large molecules such as proteins, but also smaller molecules like water. This prevents foreign molecules from entering the cell and helps regulate the internal environment of the cell. Cells infected with certain types of epithelial cancer exhibit increased PKC-α activity. This is a result of a change in the shape of the cell membrane, particularly in the areas where tight junctions exists. [http://www.annalsnyas.org/cgi/content/abstract/915/1/231. Increased Tight Junction Permeability Can Result from Protein Kinase C Activation/Translocation and Act as a Tumor Promotional Event in Epithelial Cancers. JAMES M. MULLINa, KATHLEEN V. LAUGHLIN. The Lankenau Medical Research Center.] With greater activity of PKC-α, the tight junctions lose their ability to form a tight barrier. [http://www.jbc.org/cgi/content/full/272/23/14950. “Protein Kinase C- Activity Modulates Transepithelial Permeability and Cell Junctions in the LLC-PK1 Epithelial Cell Line.” Dan Rosson §, Thomas G. O'Brien. Lankenau Medical Research Center] This causes an increased leakiness of the tight junctions and thus movement of molecules into the cells. In intestinal areas, luminal growth factors are able to enter the cell and increase the rate of cell growth. This is thought to be a promotional event that may prolong certain epithelial cancers.

Hepatic Studies

Much of the research of PKC alpha pertaining to its role in liver tissue involves the effects of bile acids on the phosphorylation mechanism of the PKC family of proteins. Past research has affirmed that the bile acid CDCA inhibits the healthy glucagon response through a phosphorylation-related sequence. In related studies further testing the effects of CDCA on hepatocytes, CDCA was shown to have induced PKC translocation to the plasma membrane. [http://ajpgi.physiology.org/cgi/content/full/291/2/G275 Bile acids stimulate PKC{alpha} autophosphorylation and activation: role in the attenuation of prostaglandin E1-induced cAMP production in human dermal fibroblasts - Am J Physiol Gastrointest Liver Physiol] Interestingly, PKC alpha was favored in this process over PKC delta. The implications of this finding are that increased interaction between the glucagon receptor and PKC alpha could occur. [ [http://endo.endojournals.org/cgi/content/full/147/11/5294 Decreased Glucagon Responsiveness by Bile Acids: A Role for Protein Kinase C{alpha} and Glucagon Receptor PhosphorylationTadashi Ikegami, Lada Krilov, Jianping Meng, Bhumika Patel, Kelli Chapin-Kennedy and Bernard Bouscarel- Endocrinology, doi:10.1210/en.2006-0516] ]

Cardiac Studies

PKC alpha is one of the lesser studied proteins of the PKC family because it is not highly regulated in the serious medical condition known as acute myocardial ischemia, which results from a lack of blood supply to the myocardium (heart muscle tissue). Recent research into the role of PKC alpha in cardiac tissue has indicated that it has an important role in stimulating hypertrophy. This was demonstrated by the ability of agonist-mediated hypertrophy to be stopped only as a result of the inhibition of PKC alpha in an experiment in situ. However, in further in vivo research using mice, the transgenic overexpression of PKC alpha showed no effect on cardiac growth, and the inhibition of PKC alpha showed no effect on hypertrophic response to increased cardiac pressure. On the contrary, research has shown that removing PKC alpha altogether improved the hearts ability to contract. [http://www.jci.org/cgi/content/full/115/3/527 Protein kinase cascades in the regulation of cardiac hypertrophyGerald W. Dorn, II and Thomas Force - J. Clin. Invest. 115:527-537 (2005). doi:10.1172/JCI200524178.]

In summary, research is pointing in the direction that PKC alpha’s role in cardiac tissue has more impact as a regulator of contractility than of hypertrophy. In another study, the binding peptides, RACK and others derived from PKC beta, were expressed in mouse hearts. The genetic code for these proteins are similar to those of all isoforms of the PKC family (alpha, beta, and gamma). As such, RACK and other proteins can regulate the expression of all PKC family proteins. In this particular study, however, only PKC alpha was affected. Again, overexpression caused decreased contractile performance, whereas inhibition saw increased performance. [http://www.jci.org/cgi/content/full/115/3/527 Protein kinase cascades in the regulation of cardiac hypertrophyGerald W. Dorn, II and Thomas Force - J. Clin. Invest. 115:527-537 (2005). doi:10.1172/JCI200524178.]

Future Research Prospects

PKC-α shows important regulation of phospholipase D. Phospholipase D is located on the plasma membrane and is responsible for hydrolyzing phosphatidylcholine to phosphatidic acid and choline. Research has indicated that phospholipase D may play roles in tumorigenesis by altering cellular events such as invasion and migration. Point mutations at particular phenylalanine residues have shown to inhibit PKC-α’s ability to activate phospholipase D. [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WBK-4GCWXVC-2&_user=655954&_coverDate=08%2F05%2F2005&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000035538&_version=1&_urlVersion=0&_userid=655954&md5=9f93a6082751229d55a96df3262a98c8 A point mutation at phenylalanine 663 abolishes protein kinase Cα’s ability to translocate to the perinuclear region and activate phospholipase D1

Tianhui Hu and John H. Exton] Current research is being conducted investigating PKC-α’s inhibitory affects. Researchers hope to learn how to exploit PKC-α’s ability to turn down phospholipase D’s activity and use this function to create anti-cancer drugs.

Another breakthrough branch of research concerning PKC-α concerns its role in erythrocyte (red blood cell) development. Currently, researchers understand that PKC-α is correlated with the differentiation of erythroid progenitor cells in bone marrow. [http://bloodjournal.hematologylibrary.org/cgi/content/full/95/2/510 Protein kinase C- isoform is involved in erythropoietin-induced erythroid differentiation of CD34+ progenitor cells from human bone marrow June Helen Myklebust, Erlend B. Smeland, Dag Josefsen, and Mouldy Sioud From the Department of Immunology, The Norwegian Radium Hospital, Oslo, Norway. ] These undifferentiated cells give rise to the mass of red blood cells present in blood. Future research endeavors seek to find whether it is activation or inhibition of PKC-α which affects the development of erythrocytes. [http://bloodjournal.hematologylibrary.org/cgi/content/full/95/2/510 Protein kinase C- isoform is involved in erythropoietin-induced erythroid differentiation of CD34+ progenitor cells from human bone marrow June Helen Myklebust, Erlend B. Smeland, Dag Josefsen, and Mouldy Sioud From the Department of Immunology, The Norwegian Radium Hospital, Oslo, Norway. ] By answering this question, scientists hope to gain insight into various types of hematologic diseases such as aplastic anemia and leukemia.

Genes Associated with PKC alpha

[http://www.gene.ucl.ac.uk/nomenclature/data/get_data.php?hgnc_id=9394 PICK1] [http://www.gene.ucl.ac.uk/nomenclature/data/get_data.php?hgnc_id=9393 PRKCA]

External links

* [http://www.genome.jp/kegg/pathway.html KEGG Pathway]
* [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pubmed PubMed]
* [http://www.gene.ucl.ac.uk/ UCL Gene Database]
* [http://www.brenda.uni-koeln.de/ BRENDA Gene Database]

References

Further reading

PBB_Further_reading
citations =
*cite journal | author=O'Brian CA |title=Protein kinase C-alpha: a novel target for the therapy of androgen-independent prostate cancer? (Review-hypothesis). |journal=Oncol. Rep. |volume=5 |issue= 2 |pages= 305–9 |year= 1998 |pmid= 9468546 |doi=
*cite journal | author=Ali A, Hoeflich KP, Woodgett JR |title=Glycogen synthase kinase-3: properties, functions, and regulation. |journal=Chem. Rev. |volume=101 |issue= 8 |pages= 2527–40 |year= 2002 |pmid= 11749387 |doi=
*cite journal | author=Slater SJ, Ho C, Stubbs CD |title=The use of fluorescent phorbol esters in studies of protein kinase C-membrane interactions. |journal=Chem. Phys. Lipids |volume=116 |issue= 1-2 |pages= 75–91 |year= 2003 |pmid= 12093536 |doi=

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