Oleosin

Oleosins are structural proteins found in oil bodies and found in plant cells. Oil bodies are not considered organelles because they have a single layer membrane and lack the pre-requisite double layer membrane in order to be considered an organelle. They are found in plant parts with high oil content that undergo extreme desiccation as part of their maturation process, and help stabilize the bodies.^[1]

Oleosins comprise of three parts. The N- and C-terminal domains are amphipathic, whereas the middle part is strongly hydrophobic. Models show oleosins having a hairpin-like hydrophobic shape that is inserted inside the triacylglyceride (TAG), while the hydrophilic parts are left outside oil bodies. ^[2]

Oleosins have been found on oil bodies of seeds, tapetum cells, and pollen but not fruits. Instead of a stabilizer of oil bodies, oleosins are believe to be involved in water-uptaking of pollen on stigma.

Use in Purification of Recombinant Protein

Oleosins provide an easy way of purifying proteins which have been produced recombinantly in plants. If the protein is made as a fusion protein with oleosin and a protease recognition site is incorporated between them, the fusion protein will sit in the membrane of the oil body, which can be easily isolated by centrifugation. The oil droplets can then be mixed with aqueous medium again, and oleosin cleaved from the protein of interest. Centrifugation will cause two phases to separate again, and the aqueous medium now contains the purified protein.

References

1. ^ Hsieh, Kai; Anthony H.C. Huang (September 2005). "Lipid-rich tapetosomes in Brassica tapetum are composed of oleosin-coated oil droplets and vesicles, both assembled in and then detached from the endoplasmic reticulum". The Plant Journal 43 (6): 889–99. doi:10.1111/j.1365-313X.2005.02502.x. PMC 1867322. PMID 17307923. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1867322. 
2. ^ oleosin

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This botany article is a stub. You can help Wikipedia by expanding it.v · Categories: Plant cells Proteins Protein stubs Botany stubs Wikimedia Foundation. 2010. Игры ⚽ Поможем решить контрольную работу Obernbreit Stella Isaacs, Marchioness of Reading Look at other dictionaries: Oleosin — Oleosine sind Strukturproteine, die man in Pflanzenzellen findet, genauer im Ölkörper, dem Oleosom. Sie werden in den Monolayer des Oleosoms integriert und verhindern das Zusammenklumpen der einzelnen Lipidtröpfchen. Sie bestehen aus drei Teilen …   Deutsch Wikipedia Erdnussallergene — Die Erdnussallergene (Ara h) sind Proteine, die in Erdnüssen (Arachis hypogaea) vorkommen und stark allergen beim Menschen wirken. Sie werden in etwa ein Dutzend Unterfamilien eingeteilt, die je nach Herkunft der Pflanze in unterschiedlichem… …   Deutsch Wikipedia SemBioSys Genetics — Inc. (tsx|SBS) is a biotechnology company focussed on developing pharmaceutical proteins, industrial reagents, food additives, protein purification technologies and drug delivery systems that take advantage of the company’s proprietary… …   Wikipedia Oil body — Not to be confused with Elaiosome, a multicellular structure found in seeds. An oil body is a lipid containing structure found in plant cells. The term can refer to at least two distinct kinds of structures in different kinds of plants. Contents… …   Wikipedia 18+ © Academic, 2000-2024 Contact us: Technical Support, Advertising Dictionaries export, created on PHP, Joomla, Drupal, WordPress, MODx. Mark and share Search through all dictionaries Translate… Search Internet Share the article and excerpts Direct link … Do a right-click on the link above and select “Copy Link”