Elution volume

Elution volume

Elution volume refers to the volume of eluent (e.g. a buffer or a solvent) used in chromatography to remove one or more compounds (e.g. an amino acid or an organic compound) from a chromatographic bed. When eluting amino acids the volume of liquid used corresponds to a particular amino acid, thus an identity of the amino acid can be established by referring to the volume of buffer used. Used after the ion-exchange chromatography run has been completed.


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