Cell fractionation

Cell fractionation

Cell fractionation is the separation of homogeneous sets, usually organelles, from a heterogeneous population of cells.

teps

#Disruption (homogenisation) of cells and liberation of organelles.
#Macro Filtration
#Purification of cell components.

Homogenization

Tissue is typically homogenised in an isotonic buffer solution using a variety of mechanisms. A 'Potter-Elvehjem homogeniser' is often used as it is relatively gentle. Other procedures include grinding, mincing, chopping, pressure changes, osmotic shock and freeze-thawing.

The solution is homogenised in an isotonic solution to stop osmotic damage, with a pH buffer to regulate pH, and at an ice-cold temperature to prevent enzyme damage.

See Cell disruption for further details.

Filtration

This step may not be necessary depending on the source of the cells. Animal tissue however is likely to yield connective tissue which must be removed. Commonly, filtration is achieved either by pouring through gauze or with a suction filter and the relevant grade ceramic filter.

Purification

Invariably achieved by Differential centrifugation - the sequential increase in gravitational force resulting in the sequential separation of organelles according to their density.

ee also

*Cell disruption
*Differential centrifugation

External links

* [http://www.microfluidicscorp.com/cell_disruption/index.html Microfluidicscorp - MFIC OTC]


Wikimedia Foundation. 2010.

Игры ⚽ Поможем сделать НИР

Look at other dictionaries:

  • cell fractionation — Strictly this should mean the separation of homogeneous sets from a heterogeneous population of cells (by a method such as flow cytometry), but the term is more frequently used to mean subcellular fractionation ie. the separation of different… …   Dictionary of molecular biology

  • Fractionation — See also: Fractionated spacecraft Fractionation is a separation process in which a certain quantity of a mixture (solid, liquid, solute, suspension or isotope) is divided up in a number of smaller quantities (fractions) in which the composition… …   Wikipedia

  • cell — cell1 cell like, adj. /sel/, n. 1. a small room, as in a convent or prison. 2. any of various small compartments or bounded areas forming part of a whole. 3. a small group acting as a unit within a larger organization: a local cell of the… …   Universalium

  • Cell biology — Not to be confused with Cell (biology). Cell research redirects here. For the journal, see Cell Research (journal) …   Wikipedia

  • fractionation — A term used to describe any method for separating and purifying biological molecules. See also cell fractionation …   Dictionary of molecular biology

  • Cell disruption — is a method or process for releasing biological molecules from inside a cell. Choice of disruption method The production of biologically interesting molecules using cloning and culturing methods allows the study and manufacture of relevant… …   Wikipedia

  • Cell synchronization — Cell Synchronization. Synchronization literally means to make two or more things happen exactly simultaneously. For instance, two or more watches can be synchronized to show exactly the same time.The cells at different stages of the cell cycle in …   Wikipedia

  • List of topics in cell biology — Cell invokes a major branch of theory and research known variously as cell biology, cellular biology or cytology. The study of cell tissues is known as histology. Cell types are often referred to using the suffixes blast, clast, cyte, especially… …   Wikipedia

  • Outline of cell biology — Light micrograph of a moss s leaf cells at 400X magnification. The following outline is provided as an overview of and topical guide to cell biology: Cell biology (formerly cytology, from the Greek kytos, container ) – academic discipline that… …   Wikipedia

  • Dose fractionation — Experiments in radiation biology have found that as the absorbed dose of radiation increases, the number of cells which survive decreases. They have also found that if the radiation is fractionated into smaller doses, with one or more rest… …   Wikipedia

Share the article and excerpts

Direct link
… Do a right-click on the link above
and select “Copy Link”