Bacterial display

Bacterial display

Bacterial Display (or bacteria display or bacterial surface display) is a protein engineering technique used for in vitro protein evolution. Libraries of polypeptides displayed on the surface of bacteria can be screened using flow cytometry or iterative selection procedures (biopanning).

Competing methods for protein evolution "in vitro" are phage display, ribosome display, yeast display, and mRNA display.

Citations

* Francisco, J. A., Campbell, R., Iverson, B.L., Georgiou, G.; Proc. Nat. Acad. Sci. U.S.A., 1993, 90, 10444-48.
* Georgiou, G., et al.; Nat. Biotech., 1997, 15, 29-34.


Wikimedia Foundation. 2010.

Игры ⚽ Нужна курсовая?

Look at other dictionaries:

  • Display — may refer to: Display (horse) (1923–1944), an American thoroughbred racehorse Display (zoology), a form of animal behaviour Display advertising, type that typically contains text, i.e., copy, logos, images, location maps, etc. Display case, also… …   Wikipedia

  • Phage display — is a method for the study of protein protein, protein peptide, and protein DNA interactions that utilizes bacteriophage to connect proteins with the genetic information that encodes them.cite journal |author=Smith GP |title= Filamentous fusion… …   Wikipedia

  • MRNA display — is a display technique used for in vitro protein, and/or peptide evolution to create molecules that can bind to a desired target. The process results in translated peptides or proteins that are associated with their mRNA progenitor via a… …   Wikipedia

  • Ribosome display — is a technique used to perform in vitro protein evolution to create proteins that can bind to a desired ligand. The process results in translated proteins that are associated with their mRNA progenitor which is used, as a complex, to bind to an… …   Wikipedia

  • Yeast display — (or yeast surface display) is a technique used in the field of protein engineering. The yeast display technique was first published by the laboratory of Professor K. Dane Wittrup. [ [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed… …   Wikipedia

  • In vitro compartmentalization — (IVC) is an emulsion based technology that generates cell like compartments in vitro . These compartments are designed such that each contains no more than one gene. When the gene is transcribed and/or translated, its products (RNAs and/or… …   Wikipedia

  • Chromatin immunoprecipitation — The procedure of chromatin immunoprecipitation (ChIP) assay and methods of analysis Chromatin Immunoprecipitation (ChIP) is a type of immunoprecipitation experimental technique used to investigate the interaction between proteins and DNA in the… …   Wikipedia

  • Green fluorescent protein — EGFP redirects here. EGFP may also refer to the ICAO airport code for Pembrey Airport. GFP ribbon diagram. From PDB 1EMA …   Wikipedia

  • Gel electrophoresis — apparatus – An agarose gel is placed in this buffer filled box and electrical field is applied via the power supply to the rear. The negative terminal is at the far end (black wire), so DNA migrates toward the camera. Classification… …   Wikipedia

  • SDS-PAGE — PAGE redirects here. For other uses, see Page (disambiguation). Picture of an SDS PAGE. The molecular marker is in the left lane SDS PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a collection of related techniques… …   Wikipedia

Share the article and excerpts

Direct link
Do a right-click on the link above
and select “Copy Link”