Phenol extraction

Phenol extraction

Phenol extraction is a method used to purify a DNA sample.

In this method, a mixture of TE (or Tris-Ethylenediaminetetraacetic acid) and phenol is combined with an equal volume of an aqueous DNA sample. After agitation and centrifugal separation, the aqueous layer is extracted, and further processed with ether. Then the DNA is concentrated by ethanol precipitation.

ee also

*Phenol-chloroform extraction

References

*http://mycoplasmas.vm.iastate.edu/lab_site/methods/DNA/phenol.html


Wikimedia Foundation. 2010.

Игры ⚽ Поможем написать реферат

Look at other dictionaries:

  • Phenol-chloroform extraction — is a liquid liquid extraction technique in biochemistry. It is widely used in molecular biology for isolating DNA, RNA and protein. Equal volumes of a phenol:chloroform mixture and an aqueous sample are mixed, forming a biphasic mixture. This… …   Wikipedia

  • Extraction de l'ADN — Extraction d ADN L extraction de l ADN est une technique permettant d isoler l ADN de cellules ou de tissus. L ADN ainsi extrait peut ensuite être utilisé pour des recherches de biologie moléculaire, telles que le séquençage, la PCR ou le clonage …   Wikipédia en Français

  • Phenol (molecule) — Phénol (molécule) Pour les articles homonymes, voir Phénol. Phénol structur …   Wikipédia en Français

  • Extraction d'ADN — L extraction de l ADN est une technique permettant d isoler l ADN de cellules ou de tissus. L ADN ainsi extrait peut ensuite être utilisé pour des recherches de biologie moléculaire, telles que le séquençage, la PCR ou le clonage. Il existe… …   Wikipédia en Français

  • Phenol-Chloroform-Extraktion — Schematische Darstellung der Phenol Chloroform Extraktion Als Phenol Chloroform Extraktion wird eine biochemische Extraktionsmethode bezeichnet, welche in der Molekularbiologie für die Trennung von Proteinen, DNA und RNA eingesetzt wird. Das… …   Deutsch Wikipedia

  • Phénol (molécule) — Pour les articles homonymes, voir Phénol. Phénol structure du phénol …   Wikipédia en Français

  • RNA extraction — is the purification of RNA from biological samples. This procedure is complicated by the ubiquitous presence of ribonuclease enzymes in cells and tissues, which can rapidly degrade RNA. [cite journal |author=Peirson SN, Butler JN |title=RNA… …   Wikipedia

  • Olive oil extraction — An olive mill and an olive press dating from Roman times in Capernaum, Israel. Olive oil extraction is the process of extracting the oil present in the olive drupes for food use. The oil is produced in the mesocarp cells, and stored in a… …   Wikipedia

  • Natural phenol — Quercetin, a typical flavonoid, is a natural phenol Natural phenols, bioavailable phenols, plant phenolics, low molecular weight phenols or phenoloids[1][2] …   Wikipedia

  • Gel extraction — In molecular biology, gel extraction or gel isolation is a technique used to isolate a desired fragment of intact DNA from an agarose gel following agarose gel electrophoresis. After extraction, fragments of interest can be mixed, precipitated,… …   Wikipedia

Share the article and excerpts

Direct link
Do a right-click on the link above
and select “Copy Link”