Nitrate reductase test

The nitrate reductase test is a test to differentiate between bacteria based on their ability or inability to reduce nitrate (NO₃⁻) to nitrite (NO₂⁻) using anaerobic respiration.

Procedure

Various assays for detecting nitrate reduction have been described.^[1] One method is performed as follows:

1. Inoculate nitrate broth with an isolate and incubate for 48 hours.
2. Add 10-15 drops each of sulfanilic acid and N,N-dimethyl-1-naphthylamine. If the bacterium produces nitrate reductase, the broth will turn a deep red within 5 minutes at this step.
3. If no color change is observed, then the result is inconclusive. Add a small amount of zinc to the broth. If the solution remains colorless, then both nitrate reductase and nitrite reductase are present. If the solution turns red, nitrate reductase is not present.

References

1. ^ V.B.D. Skerman, A guide to the identification of the genera of bacteria, The Williams & Wilkins Co., Baltimore, MD, p.218 - 220 (1967).