Diagnostic electron microscopy

The transmission electron microscope (TEM) is used as an important diagnostic tool to screen human tissues at high magnification (the ultrastructural level), often in conjunction with other methods, particularly light microscopy and immunofluorescence techniques. The TEM was first used extensively for this purpose in the 1980s, especially for identifying the markers of cell differentiation to identify tumours, and in renal disease. Immunolabelling techniques are now generally used instead of the TEM for tumour diagnosis but the technique retains a critical role in the diagnosis of renal disease and a range of other conditions.

Specifically, TEM should be used for diagnostic purposes when it: (1) provides useful (complementary) information in the context of a carefully considered differential diagnosis; (2) provides an ‘improved’ diagnosis that results in better treatment strategies and; (3) is time & cost effective in respect to alternative techniques. For diagnostic purposes solid tissues are prepared for TEM in the same way as other biological tissues, they are fixed in glutaraldehyde and osmium tetroxide then dehydrated and embedded in epoxy resin. The epoxy resin block is trimmed and the target tissue is selected using a light microscope by viewing semithin sections stained with toluidine blue. The block is then retrimmed and the specific area for observation is ultrathin sectioned, preferably using a diamond knife. The ultrathin sections are collected on 3mm copper (mesh) grids and stained with uranyl acetate and lead citrate to make the contents of the tissue electron dense (and thus visible in the electron microscope).^[1]

References

1. ^ Woods AE, Stirling JW. 2008. Electron microscopy. In, Theory and Practice of Histological Techniques. Eds, Bancroft JD and Gamble M. 6th edition. Churchill Livingstone: pages 601-640

External links

The Association of Clinical Electron Microscopists (UK)[1]