- Chondroitin B lyase
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Chondroitin B lyase Identifiers EC number 4.2.2.19 CAS number 52227-83-5 Databases IntEnz IntEnz view BRENDA BRENDA entry ExPASy NiceZyme view KEGG KEGG entry MetaCyc metabolic pathway PRIAM profile PDB structures RCSB PDB PDBe PDBsum Search PMC articles PubMed articles In enzymology, a chondroitin B lyase (EC 4.2.2.19) is an enzyme that catalyzes the chemical reaction
- Eliminative cleavage of dermatan sulfate containing 1,4-beta-D-hexosaminyl and 1,3-beta-D-glucurosonyl or 1,3-alpha-L-iduronosyl linkages to disaccharides containing 4-deoxy-beta-D-gluc-4-enuronosyl groups to yield a 4,5-unsaturated dermatan-sulfate disaccharide (deltaUA-GalNAc-4S).
This enzyme belongs to the family of lyases, specifically those carbon-oxygen lyases acting on polysaccharides. The systematic name of this enzyme class is chondroitin B lyase. Other names in common use include chondroitinase B, ChonB, and ChnB.
References
- Gu K, Linhardt RJ, Laliberte M, Gu K, Zimmermann J (Pt 2). "Purification, characterization and specificity of chondroitin lyases and glycuronidase from Flavobacterium heparinum". Biochem. J. 312 (Pt 2): 569–77. PMC 1136300. PMID 8526872. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1136300.
- Pojasek K, Raman R, Kiley P, Venkataraman G, Sasisekharan R (2002). "Biochemical characterization of the chondroitinase B active site". J. Biol. Chem. 277 (34): 31179–86. doi:10.1074/jbc.M201552200. PMID 12063249.
- Pojasek K, Shriver Z, Kiley P, Venkataraman G, Sasisekharan R (2001). "Recombinant expression, purification, and kinetic characterization of chondroitinase AC and chondroitinase B from Flavobacterium heparinum". Biochem. Biophys. Res. Commun. 286 (2): 343–51. doi:10.1006/bbrc.2001.5380. PMID 11500043.
- Suzuki K, Terasaki Y, Uyeda M (2002). "Inhibition of hyaluronidases and chondroitinases by fatty acids". J. Enzyme. Inhib. Med. Chem. 17 (3): 183–6. doi:10.1080/14756360290032930. PMID 12443044.
- Ototani N, Yosizawa Z (1979). "Purification of chondroitinase B and chondroitinase C using glycosaminoglycan-bound AH-Sepharose 4B". Carbohydr. Res. 70 (2): 295–306. doi:10.1016/S0008-6215(00)87109-8. PMID 427837.
- K, Zimmermann JJ, Su H (2000). "Isolation and Expression in Escherichia coli of cslA and cslB, Genes Coding for the Chondroitin Sulfate-Degrading Enzymes Chondroitinase AC and Chondroitinase B, Respectively, from Flavobacterium heparinum". Appl. Environ. Microbiol. 66 (1): 29–35. doi:10.1128/AEM.66.1.29-35.2000. PMC 91781. PMID 10618199. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=91781.
- V, Sasisekharan R, Cygler M (2004). "The structure of chondroitin B lyase complexed with glycosaminoglycan oligosaccharides unravels a calcium-dependent catalytic machinery". J. Biol. Chem. 279 (31): 32882–96. doi:10.1074/jbc.M403421200. PMID 15155751.
- Li Y, Matte A, Su H, Cygler M (1999). "Crystallization and preliminary X-ray analysis of chondroitinase B from Flavobacterium heparinum". Acta Crystallogr. D 55 (Pt 5): 1055–7. doi:10.1107/S0907444999002097. PMID 10216304.
- Huang W, Matte A, Li Y, Kim YS, Linhardt RJ, Su H, Cygler M (1999). "Crystal structure of chondroitinase B from Flavobacterium heparinum and its complex with a disaccharide product at 1.7 A resolution". J. Mol. Biol. 294 (5): 1257–69. doi:10.1006/jmbi.1999.3292. PMID 10600383.
- Lauder RM; Nieduszynski, IA; Giannopoulos, M; Weeks, SD; Sadler, IH; Lauder, RM (2005). "Characterization of oligosaccharides from the chondroitin/dermatan sulfates. 1H-NMR and 13C-NMR studies of reduced trisaccharides and hexasaccharides". Febs. J. 272 (24): 6276–86. doi:10.1111/j.1742-4658.2005.05009.x. PMID 16336265.
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