Isopropyl β-D-1-thiogalactopyranoside
- Isopropyl β-D-1-thiogalactopyranoside
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ImageFile = IPTG.png ImageSize =
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Section1 = Chembox Identifiers
CASNo = 367-93-1
PubChem = 656894
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MeSHName = Isopropyl+Thiogalactoside
Section2 = Chembox Properties
Formula = C9H18O5S
MolarMass = 238.302
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Isopropyl β-D-1-thiogalactopyranoside, abbreviated IPTG, is a molecular biology reagent.
This compound is used as a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the "lac" operon. Unlike allolactose, the sulfur (S) atom creates a chemical bond which is non-hydrolyzable by the cell, preventing the cell from "eating up" or degrading the inductant; therefore the IPTG concentration remains constant.
IPTG induces the transcription of the gene coding for beta-galactosidase, an enzyme that promotes lactose utilization, by binding and inhibiting the LacI repressor. In cloning experiments, the "lacZ" gene is replaced with the gene of interest and IPTG is then used to induce gene expression.
Many regulatory elements of the "lac" operon are used in inducible recombinant protein systems; IPTG is an effective inducer in the concentration range of 100 to 1.5 .
See also
X-gal
External links
* [http://www.acros.com/DesktopModules/Acros_Search_Results/Acros_Search_Results.aspx?search_type=CatalogSearch&SearchString=iptg IPTG's description on the Acros website]
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