Transformation efficiency
- Transformation efficiency
Transformation efficiency is calculated by dividing the number of successful transformants by the amount of DNA used during a transformation procedure. Transformants are cells which have taken up DNA (foreign, artificial or modified) and which can express genes on the introduced DNA.
Factors affecting transformation efficiency
A study done in "E. coli" found that transformation efficiency declines linearly with increasing plasmid size, and that there is a similar transformation efficiency for relaxed and supercoiled plasmids. This study also found that individual cells were capable of taking up many DNA molecules, and that the presence of multiple plasmids did not significantly affect the occurrence of successful transformation events. [cite journal |author=Hanahan D |title=Studies on the transformation of "Escherichia coli" with plamids |journal=J Mol Bio |volume=166 |issue=4 |pages=557–580 |year=1983 |pmid=6345791 |doi=10.1016/S0022-2836(83)80284-8]
See also
* Transformation (genetics)
References
External links
[http://www.sciencegateway.org/tools/transform.htm Bacteria Transformation Efficiency Calculator]
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