RyhB RNA

RyhB RNA is a 90 nucleotide non-coding RNA that down-regulates a set of iron-storage and iron-using proteins when iron is limiting; it is itself negatively regulated by the ferric uptake repressor protein, Fur (Ferric uptake regulator). This ncRNA gene was recently identified in a screen and called SraI and was found to be expressed only in stationary phase. [cite journal | last = Argaman | first = L | coauthors = Hershberg R, Vogel J, Bejerano G, Wagner EG, Margalit H, Altuvia S | year = 2001 | title = Novel small RNA-encoding genes in the intergenic regions of Escherichia coli | journal = Curr Biol | volume = 11 | pages = 941–950 | pmid = 11448770 | doi = 10.1016/S0960-9822(01)00270-6] RyhB RNA levels are inversely correlated with mRNA levels for the sdhCDAB operon, encoding succinate dehydrogenase, as well as five other genes previously shown to be positively regulated by Fur by an unknown mechanism. These include two other genes encoding enzymes in the tricarboxylic acid cycle, acnA and fumA, two ferritin genes, ftnA and bfr, and a gene for superoxide dismutase, sodB. [cite journal | last = Masse | first = E | coauthors = Gottesman S | year = 2002 | title = A small RNA regulates the expression of genes involved in iron metabolism in Escherichia coli | journal = Proc Natl Acad Sci U S A | volume = 99 | pages = 4620–4625 | pmid = 11917098 | doi = 10.1073/pnas.032066599] A number of other genes have been predicted computationally and verified as targets by microarray analysis: napF, sodA, cysE, yciS, acpS, nagZ and dadA.cite journal | author = Tjaden B, Goodwin SS, Opdyke JA, "et al" | title = Target prediction for small, noncoding RNAs in bacteria | journal = Nucleic Acids Res. | volume = 34 | issue = 9 | pages = 2791–802 | year = 2006 | pmid = 16717284 | doi = 10.1093/nar/gkl356 | issn = ] RyhB is bound by the Hfq protein, that increases its interaction with its target messages.

References

* [4] cite journal | last = Gottesman | first = S | year = 2005 | title = Micros for microbes: non-coding regulatory RNAs in bacteria | journal = Trends Genet | volume = 21 | pages = 399–404 | pmid = 15913835 | doi = 10.1016/j.tig.2005.05.008

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