- RNAI
RNAI is a
non-coding RNA that is an antisense repressor of the replication of some "E. coli " plasmids, including ColE1. Plasmid replication is usually initiated by RNAII, which acts as a primer by binding to its templateDNA . The complementary RNAI binds RNAII prohibiting it from its initiation role. The rate of degradation of RNAI is therefore a major factor in control of plasmid replication. This rate of degradation is aided by the pcnB (plasmid copy number B) gene product [cite journal | last = He | first = L | coauthors = Soderbom F, Wagner EG, Binnie U, Binns N, Masters M | year = 1993 | title = PcnB is required for the rapid degradation of RNAI, the antisense RNA that controls the copy number of ColE1-related plasmids | journal = Mol Microbiol | volume = 9 | pages = 1131–1142 | pmid = 7523833 | doi = 10.1111/j.1365-2958.1993.tb01243.x] , which polyadenylates the 3' end of RNAI targeting it for degradation by PNPase. [cite journal | last = Xu | first = F | coauthors = Lin-Chao S, Cohen SN | year = 1993 | title = The Escherichia coli pcnB gene promotes adenylylation of antisense RNAI of ColE1-type plasmids in vivo and degradation of RNAI decay intermediates | journal = Proc Natl Acad Sci U S A | volume = 90 | pages = 6756–6760 | pmid = 7688127 | doi = 10.1073/pnas.90.14.6756]References
*
External links
*
Wikimedia Foundation. 2010.
