- QUAD RNA
QUAD RNAs refers to a group of related
non-coding RNA (ncRNA) identified in the bacteria "Escherichia coli " but their function is unknown.These small RNA were identified computationally by searching the genome of "E. coli" for
intergenic regions of high sequence identity (sequence conservation) with the genomes of closely related bacteria (severalsalmonella species and "Klebsiella pneumoniae ")cite journal | author = Wassarman KM, Repoila F, Rosenow C, Storz G, Gottesman S | title = Identification of novel small RNAs using comparative genomics and microarrays | journal = Genes Dev. | volume = 15 | issue = 13 | pages = 1637–51 | year = 2001 | pmid = 11445539 | doi = 10.1101/gad.901001 | issn = ] . This data was combined withmicroarray expression analysis and potential novel ncRNAs identified. The expression of novel ncRNA of interest was confirmed bynorthern blotting .In this large scale screen these ncRNAs were simply referred to as candidates 43,55 and 61. These 3 ncRNA appear to be highly homologous and are derived from a repeat region of the genome. These RNA were identified in another study cite journal | author = Rudd KE | title = Novel intergenic repeats of Escherichia coli K-12 | journal = Res. Microbiol. | volume = 150 | issue = 9-10 | pages = 653–64 | year = 1999 | pmid = 10673004 | doi = | issn = ] Each of the ncRNA contains a short stretch homologous to boxC, a repeat element of unknown function present in 50 copies or more within the genome of "E. coli" [ Bachellier, S., Gilson, E., Hofnung, M., and Hill, C.W. 1996. Repeated sequences. In Escherichia coli and Salmonella: Cellular and molecular biology (ed. F.C. Neidhardt, et al.), pp. 2012-2040. American Society for Microbiology, Washington, D.C.]
References
External links
*
Wikimedia Foundation. 2010.
