Chromosome jumping

Chromosome jumping

Chromosome jumping is a tool of molecular biology that is used in the physical mapping of genomes. It is related to several other tools used for the same purpose, including chromosome walking.

Chromosome jumping is used to bypass regions difficult to clone, such as those containing repetitive DNA, that cannot be easily mapped by chromosome walking, and is useful in moving along a chromosome rapidly in search of a particular gene.

In chromosome jumping, the DNA of interest is identified, cut into fragments with restriction enzymes, and circularised (the beginning and end of each fragment are joined together to form a circular loop). From a known sequence a primer is designed to sequence across the circularised junction. This primer is used to jump 100 kb-300 kb intervals: a sequence 100 kb away would have come near the known sequence on circularisation. Thus, sequences not reachable by chromosome walking can be sequenced. Chromosome walking can be used from the new jump position (in either direction) to look for gene-like sequences, or additional jumps can be used to progress further along the chromosome.



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  • chromosome jumping — chromosome jumping. См. прыжки по хромосоме. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • chromosome jumping — A technique that allows two segments of duplex DNA that are separated by thousands of base pairs (about 200 kb) to be cloned together. After sub cloning, each segment can be used as a probe to identify cloned DNA sequences that, at the chromosome …   Glossary of Biotechnology

  • jumping library — See: chromosome jumping …   Glossary of Biotechnology

  • jumping gene — noun a segment of DNA that can become integrated at many different sites along a chromosome (especially a segment of bacterial DNA that can be translocated as a whole) • Syn: ↑transposon • Hypernyms: ↑deoxyribonucleic acid, ↑desoxyribonucleic… …   Useful english dictionary

  • Y chromosome — Human Y chromatid The Y chromosome is one of the two sex determining chromosomes in most mammals, including humans. In mammals, it contains the gene SRY, which triggers testis development if present. The human Y chromosome is composed of about 60 …   Wikipedia

  • прыжки по хромосоме — chromosome jumping “прыжки по хромосоме”. Один из вариантов метода “прогулок по хромосоме” <chromosome walking>, отличающийся тем, что в результате какой либо мутации маркерный ген, используемый для скрининга библиотеки <genomic… …   Молекулярная биология и генетика. Толковый словарь.

  • прогулка по хромосоме — chromosome walking “прогулка по хромосоме”. Метод выделения и анализа нуклеотидных последовательностей (новых генов), фланкирующих известные гены, для которых имеются олигонуклеотидные зонды; после выделения гена с фланкирующими его… …   Молекулярная биология и генетика. Толковый словарь.

  • Primer walking — is a sequencing method of choice for sequencing DNA fragments between 1.3 and 7 kilobases. Such fragments are too long to be sequenced in a single sequence read using the chain termination method. This method works by dividing the long sequence… …   Wikipedia

  • Прыжки по хромосоме — * скачкі па храмасоме * chromosome jumping один из вариантов метода прогулки по хромосоме (см.), который отличается тем, что в результате к. л. мутации маркерный ген, используемый для скрининга геномной библиотеки (см.), перемещается по геному на …   Генетика. Энциклопедический словарь

  • Disease — Illness or sickness often characterized by typical patient problems (symptoms) and physical findings (signs). Disruption sequence: The events that occur when a fetus that is developing normally is subjected to a destructive agent such as the… …   Medical dictionary

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