Electrochromatography

Electrochromatography

Electrochromatography is a chemical separation technique in analytical chemistry, biochemistry and molecular biology used to resolve and separate mostly large biomolecules such as proteins. It is a combination of size exclusion chromatography (gel filtration chromatography) and gel electrophoresis. These separation mechanisms operate essentially in superposition along the length of a gel filtration column to which an axial electric field gradient has been added. The molecules are separated by size due to the gel filtration mechanism and by electrophoretic mobility due to the gel electrophoresis mechanism. Additionally there are secondary chromatographic solute retention mechanisms. [ [http://dx.doi.org/10.1021/ac60054a001 Analysis by Electromigration plus Chromatography] Strain, H.H., Sullivan, .J. C., Anal.Chem. 23, 816 (1951)] [ [http://dx.doi.org/10.1016/0021-9673(94)01276-K Electrochromatography of Proteins] , Basak, S., A. Velayudhan, K. Kohlmann and M. R. Ladisch, Journal of Chromatography A., 707, 69-76 (1995)]

ee also

* Chromatography
* Size exclusion chromatography
* Gel electrophoresis
* Protein electrophoresis
* Electrofocusing
* Two-dimensional gel electrophoresis
* Temperature gradient gel electrophoresis

References


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