Ki-67 (biology)

The Ki-67 protein (also known as antigen identified by monoclonal antibody Ki-67or MKI67) is a cellular marker for proliferation. It is strictly associated with cell proliferation. During the interphase, the Ki-67 antigen can be exclusively detected within the cell nucleus, whereas in mitosis most of the protein is relocated to the surface of the
chromosomes. Ki-67 protein is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0). Ki-67 is an excellent marker to determine the growth fraction of a given cell population. The fraction of Ki-67-positive tumor cells (the "Ki-67 labelling index") is often correlated with the clinical course of cancer. The best-studied examples in this context are carcinomas of the prostate and the breast. For these types of tumors, the prognostic value for survival and tumor recurrence have repeatedly been proven in uni- and multivariate analysis.

The Ki-67 protein was originally defined by the prototype monoclonal antibody Ki-67 [cite journal |author=Gerdes J, Schwab U, Lemke H, Stein H |title=Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation |journal=Int. J. Cancer |volume=31 |issue=1 |pages=13–20 |year=1983 |pmid=6339421 |url=http://www3.interscience.wiley.com/cgi-bin/abstract/112713361/ |doi=10.1002/ijc.2910310104] , which was generated by immunizing mice with nuclei of the Hodgkin lymphoma cell line L428. The name is derived from the city of origin (Kiel) and the number of the original clone in the 96-well plate.

MIB-1 is a commonly used monoclonal antibody that detects the Ki-67 antigen. It is used in clinical applications to determine the "Ki-67 labelling index". One of its primary advantages over the original Ki-67 antibody (and the reason why it has essentially supplanted the original antibody for clinical use) is that it can be used on formalin-fixed paraffin-embedded sections, after heat-mediated antigen retrieval.

See also

*PCNA - Proliferating Cell Nuclear Antigen, expressed during the DNA synthesis.

References

Literature

*cite journal |author=Scholzen T, Gerdes J |title=The Ki-67 protein: from the known and the unknown |journal=J. Cell. Physiol. |volume=182 |issue=3 |pages=311–22 |year=2000 |pmid=10653597 |url=http://www3.interscience.wiley.com/cgi-bin/abstract/69501961/ |doi=10.1002/(SICI)1097-4652(200003)182:3<311::AID-JCP1>3.0.CO;2-9 |doilabel=10.1002/(SICI)1097-4652(200003)182:3311::AID-JCP13.0.CO;2-9 (Review)

External links

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