Tris-Buffered Saline

Tris-Buffered Saline (abbreviated TBS) is a buffer used in some biochemical techniques to maintain the pH within a relatively narrow range.Tris (with HCl) has a slightly alkaline buffering capacity in the 7 - 9.2 range.

Contents of TBS

The following is a sample recipe for TBS:
*20 mM Tris
*150 mM NaClAdjust pH with HCl to pH 7.6 [ [http://www.biochemj.org/bj/378/1023/bj3781023.htmhttp://www.biochemj.org/bj/378/1023/bj3781023.htm Glycoproteins VI and Ib-IX-V stimulate tyrosine phosphorylation of tyrosine kinase Syk and phospholipase Cg2 at distinct sites - Katsue SUZUKI-INOUE et al] ]

Note that concentration of Tris and NaCl as well as the pH can vary, and the resulting solution would still be referred to as "TBS". Tris concentration (giving buffering capacity) can vary from 10 mM to 100 mM. As TBS is used to emulate physiological conditions (as in animal or human body), the pH value is slightly alkaline, ranging from 7.4 - 8.0. NaCl gives the isotonic (mostly used 150Mm NaCl corresponds to physiological conditions: 0.9% NaCl) or hypertonic (500mM NaCl) salt concentration.

References