Lysis buffer

Lysis buffer

A lysis buffer is used for the purpose of lysing cells for use in experiments that analyze the compounds of the cells (e.g. western blot). There are many different kind of lysis buffers that one can apply, depending on what analysis the cell lysate will be used for e.g. red blood cell lysis buffer.

In studies like DNA fingerprinting the lysis buffer is used for DNA isolation. Dish soap can be used in a pinch to break down the cell and nuclear membranes, allowing the DNA to be released. Other such lysis buffers include Buffer P2.


Wikimedia Foundation. 2010.

Игры ⚽ Поможем решить контрольную работу

Look at other dictionaries:

  • Buffer — can refer to: * Buffer state, a country lying between two potentially hostile greater powers, thought to prevent conflict between them * Buffer zone, any area that keeps two or more other areas distant from one another, may be demilitarized *… …   Wikipedia

  • Buffer P2 — In biology, buffer P2 is a lysis buffer solution containing sodium dodecyl sulfate (SDS) to puncture holes in cellular membranes. It is used in conjunction with other resuspension buffers and lysis buffers to release DNA from cells …   Wikipedia

  • RIPA buffer — is a lysis buffer used to lyse cells and tissue, for radio immunoprecipitation assay (RIPA).RIPA buffer recipe for total protein extract is as follows:* Tris 50mM * NaCl 150 mM * SDS 0.1 % * Na.Deoxycholate 0.5 % * Triton X 100 or NP40 1% *… …   Wikipedia

  • Alkaline lysis — is a method used in molecular biology to break cells open to isolate plasmid DNA or other cell components such as proteins. Bacteria containing the plasmid of interest is first grown, then lysed with a strong alkaline buffer consisting of a… …   Wikipedia

  • Triton X-100 — Other names polyethylene glycol p (1,1,3,3 tetramethylbutyl) phenyl ether, octyl phenol ethoxylate, polyoxyethylene octyl phenyl ether, 4 oct …   Wikipedia

  • List of biochemistry topics — This page aims to list articles on Wikipedia that are related to biochemistry. This is so that those interested in the subject can monitor changes to the pages by clicking on .This list is not necessarily complete or up to date if you see an… …   Wikipedia

  • P2 — P2, P02, P.2, or P 2 might refer to several subjects: Contents 1 Technology 2 Science 3 Aircraft …   Wikipedia

  • Protein microarray — A protein microarray, sometimes referred to as a protein binding microarray, is a piece of glass on which different molecules of protein have been affixed at separate locations in an ordered manner thus forming a microscopic array. These are used …   Wikipedia

  • HLB — hydrophilic lipophilic balance; hypotonic lysis buffer …   Medical dictionary

  • HLB — • hydrophilic lipophilic balance; • hypotonic lysis buffer …   Dictionary of medical acronyms & abbreviations

Share the article and excerpts

Direct link
Do a right-click on the link above
and select “Copy Link”