RIPA buffer

RIPA buffer

RIPA buffer is a lysis buffer used to lyse cells and tissue, for radio immunoprecipitation assay (RIPA).

RIPA buffer recipe for total protein extract is as follows:

* Tris 50mM
* NaCl 150 mM
* SDS 0.1 %
* Na.Deoxycholate 0.5 %
* Triton X 100 or NP40 1%
* Protease inhibitors such as PMSF or cocktail protease inhibitors which are commercially available (use according to the manufacturer's guide)

External links

* [http://www.piercenet.com/Objects/View.cfm?type=ProductFamily&id=9984522F-D74A-4E46-829C-C057D8C21050]
* [http://www.cellsignal.com/products/9806.html]
* [http://carmen.salk.edu/~sefton/Hyper_protocols/immunoprecip.html#anchor1446740]
* [http://www.molecularstation.com/protein/immunoprecipitation/]


Wikimedia Foundation. 2010.

Игры ⚽ Нужно сделать НИР?

Look at other dictionaries:

  • Royal Military Police — logo Active 28 November 1946 Country UK …   Wikipedia

  • arts, East Asian — Introduction       music and visual and performing arts of China, Korea, and Japan. The literatures of these countries are covered in the articles Chinese literature, Korean literature, and Japanese literature.       Some studies of East Asia… …   Universalium

  • Europe, history of — Introduction       history of European peoples and cultures from prehistoric times to the present. Europe is a more ambiguous term than most geographic expressions. Its etymology is doubtful, as is the physical extent of the area it designates.… …   Universalium

Share the article and excerpts

Direct link
… Do a right-click on the link above
and select “Copy Link”