Second Generation Multiplex Plus
- Second Generation Multiplex Plus
Second Generation Multiplex Plus (SGM Plus), manufactured by ABI (Applied Biosystems), is an updated version of the Second Generation Multiplex DNA profiling system. This system has been used by The UK National DNA Database from 1998 onwards.
SGM Plus uses the same STR loci as in the original SGM DNA profiling system, though it uses different DNA primers. It also contains DNA primers for an additional four STR loci (D3, D16, D2 and D19).
SGM Plus also contains primers for the Amelogenin (Amelo) sex-indicating test.
Dye tags
The primers are tagged with the following fluorescent dyes for detection under electrophoresis:
*5-FAM
*JOE
*NED
The primers for each locus are arranged on the dyes in the following order, from low molecular weight to large molecular weight:
*5 - FAM. D3, VWA, D16, D2
*JOE. Amelo, D8, D21, D18
*NED. D19, THO, FGA
The dyes to which each primer is attached differ from those of the original SGM DNA profiling system.
Wikimedia Foundation.
2010.
Look at other dictionaries:
Second generation (disambiguation) — Second generation may refer to:* the generation following the one preceding it* History of video game consoles (second generation) (1976 1984) * Second generation immigrants ** Nisei, one of the second generation of people of Japanese descent in… … Wikipedia
Low copy number — (LCN) is a DNA profiling technique developed by the Forensic Science Service (FSS) and in use in some countries since 1999. In the United Kingdom the technique was briefly suspended from use between 21 December 2007 and 14 January 2008 while the… … Wikipedia
Murder of Lynette White — The murder of Lynette White was committed on Saint Valentine s Day, 1988 in Cardiff, Wales. Twenty year old White, a prostitute, was stabbed over fifty times in her flat above a bookmaker s at 7 James Street, Butetown.[1] In March 1988 South… … Wikipedia
performing arts — arts or skills that require public performance, as acting, singing, or dancing. [1945 50] * * * ▪ 2009 Introduction Music Classical. The last vestiges of the Cold War seemed to thaw for a moment on Feb. 26, 2008, when the unfamiliar strains … Universalium
Darwin–Wedgwood family — The Darwin–Wedgwood family is actually two interrelated English families, descended from the prominent 18th century doctor, Erasmus Darwin, and Josiah Wedgwood, founder of the pottery firm, Josiah Wedgwood and Sons, the most notable member of… … Wikipedia
Financial Times — The 19 November 2010 front page of the UK edition of the Financial Times Type Daily newspaper Format Broadsheet Owner … Wikipedia
Digital radio — has several meanings: 1. Today the most common meaning is digital radio broadcasting technologies, such as the digital audio broadcasting (DAB) system, also known as Eureka 147. In these systems, the analog audio signal is digitized into zeros… … Wikipedia
Nortel — Networks Corporation Type Public OTC Markets Group: NRTLQ Industry Telecommunications … Wikipedia
Télévision numérique terrestre en France — métonymie TNT, un type de diffusion de télév … Wikipédia en Français
Alteon — Nortel Logo de Nortel Networks Corporation Création 1895 à Montréal … Wikipédia en Français
