- Chromatin Structure Remodeling (RSC) Complex
complex and is essential for mitotic growth.cite journal | author = Cairns BR, Lorch Y, Li Y, Zhang M, Lacomis L, Erdjument-Bromage H, Tempst P, Du J, Laurent B, Kornberg RD | title = RSC, an essential, abundant chromatin-remodeling complex | journal = Cell | volume = 87 | issue = 7 | pages = 1249–60 | year = 1996 | pmid = 8980231 | doi = 10.1016/S0092-8674(00)81820-6 ]
Generation of loops in dsDNA
A single molecule study using
magnetic tweezers has observed that RSC generates DNA loops in vitro while simultaneously generating negative supercoils in the template.cite journal | author = Lia G, Praly E, Ferreira H, Stockdale C, Tse-Dinh YC, Dunlap D, Croquette V, Bensimon D, Owen-Hughes T | title = Direct observation of DNA distortion by the RSC complex | journal = Mol. Cell | volume = 21 | issue = 3 | pages = 417–25 | year = 2006 | pmid = 16455496 | doi = 10.1016/j.molcel.2005.12.013 ] The figure above shows three AFM images from that study. On the left, a single RSC complex is bound to the end of a dsDNA template. The center image shows a DNA loop generated by RSC. The image on the right shows a loop generated by RSC which containssupercoils .The current model of translocation on dsDNA is shown in the figure to the right, whereby RSC binds the DNA at two locations. Hydrolysis of ATP allows the complex to translocate the DNA into a loop. RSC can release the loop either by translocating back to the original state at a comparable velocity, or by losing one of its two contacts.
RSC components
The following is list of RSC components that have been identified in yeast and the corresponding human
ortholog s:See also
*
SWI/SNF Nucleosome Remodeling Complex
*Transcription coregulator References
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