Contig

Contig
This article is about contig in DNA sequencing. For the contig defragmentation program, see Contig (defragmentation utility).

In shotgun DNA sequencing projects, a contig (from contiguous) is a set of overlapping DNA segments derived from a single genetic source. A contig in this sense can be used to deduce the original DNA sequence of the source. This meaning of contig is in accordance with the original definition by Staden (1979).[1]

A contig map depicts the relative order of a linked library of contigs representing a complete chromosome segment.

Contents

Semantic drift

The word contig is sometimes used to refer to one segment of a contig in the original sense.

A contig is also sometimes defined as the DNA sequence reconstructed from a set of overlapping DNA segments.

Fragmentation of a contig into 1-2kb pair segments provides suitably sized DNA segments for sequencing.

A computer program is used to assemble the segments back to one single un-interrupted piece (contig).

Also a single gene or marker that identifies the comparable region in a target genome, but does not cluster with other genes or markers to form a segment is called a singleton. EST contains both contigs as singletons.

See also

  • Staden Package

References

  1. ^ Staden R (1979) A strategy of DNA sequencing employing computer programs, Nucleic Acids Research 7: 2601-2610.

External links


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