Bis-tris propane

Chembox new
ImageFile = Bis-tris propane.svg
ImageName = Skeletal formula of bis-tris propane
ImageFile1 = Bis-tris-propane-3D-balls.png ImageName1 = Ball-and-stick model of bis-tris propane
IUPACName = 2,2'-(Propane-1,3-
diyldiimino)bis [2-(hydroxymethyl)propane-1,3-diol]
Section1 = Chembox Identifiers
CASNo = 64431-96-5
SMILES = OCC(CO)(CO)NCCCNC(CO)(CO)CO
Section2 = Chembox Properties
Formula = C₁₁H₂₆N₂O₆
MolarMass = 282.34 g/mol
Density =
MeltingPt = 164-165 °C

Bis-tris propane, or 1,3-bis(tris(hydroxymethyl)methylamino)propane, is a chemical substance that is used in buffer solutions. It is a white to off-white crystalline powder that is soluble in water. It has a wide buffering range, from 6 to 9.5 due to its two pK_a values which are close in value. This buffer is primarily used in biochemistry and molecular biology.

Applications

A review of DNA polymerase fidelity cites bis-tris propane as a suitable buffer for polymerase chain reaction (PCR). [cite journal | author = Eckert K.A., and Kunkel, T.A. | title = DNA polymerase fidelity and the polymerase chain reaction | journal = PCR Methods Appl | volume = 1 | issue = 1 | pages = 17–24] Bis-Tris propane has also been used with HCl buffer for stabilization of farnesyl diphosphate isolated from a strain of Saccharomyces cerevisiae. [cite journal | author = Song, L. | title = Detection of farnesyl diphosphate accumulation in yeast ERG9 mutants | journal = Anal. Biochem. | volume = 317 | issue = 2 | pages = 180–185 | doi = 10.1016/S0003-2697(03)00138-6 | year = 2003] It has also been used in a study of the effects of buffer identity on electric signals of light-excited bacteriorhodospin. [cite journal | author = Toth-Boconadi, R., et al. | title = Buffer effects on electric signals of light-excited bacteriorhodospin | journal = Biophys. J. | volume = 78 | issue = 6 | pages = 3170–3177] Use of Bis-Tris propane has also been documented in an investigation of the MgATPase activity of the myosin subfragment 1 monomer. [cite journal | author = Bachouchi, N., | title = MgATPase activity of myosin subfragment 1. The dimer is more active than the monomer | journal = J. Mol. Biol. | volume = 191 | issue = 2 | pages = 247–254 | doi = 10.1016/0022-2836(86)90261-5 | year = 1986] The effect of buffer identity on the kinetics of the restriction enzyme EcoRV has been studied in various buffers, including Bis-Tris propane. [cite journal | author = Wenner, J.R., and Bloomfield, V.A. | title = Buffer effects on EcoRV kinetics as measured by fluorescent staining and digital imaging of plasmid cleavage | journal = Anal. Biochem. | volume = 268 | issue = 2 | pages = 201–212 | doi = 10.1006/abio.1998.3079 | year = 1999]

ee also

*Bis-tris methane
*Tris
*Tricine

References