NrrF RNA

NrrF RNA
Predicted secondary structure of NrrF RNA
Identifiers
Symbol	NrrF
Rfam	RF01416
Other data
RNA type	gene, antisense RNA
Domain(s)	Neisseria

NrrF is a non-coding RNA which is regulated by the Ferric uptake regulator (Fur) protein in bacteria. This non-coding RNA was identified in Neisseria meningitidis and is involved in iron regulation of the succinate dehydrogenase genes sdhA and sdhC. ^[1][2] NrrF acts as an antisense RNA and is complementary to the junction between the second and third genes of the sdh operon (sdhD and sdhA). Secondary structure predictions have indicated that this interaction occurs in a single stranded loop region of the NrrF RNA. Under low iron concentration NrrF is present at a high concentration and forms a duplex with the transcript in Hfq dependent manner. The RNA chaperone Hfq acts to enhance binding of NrrF or stabilizes the NrrF/sdh transcript duplex. Binding of NrrF results in down regulation of the sdhCDAB mRNA transcript results in a Fur-dependent positive regulation of succinate dehydrogenase.

NrrF appears to be restricted to the Neisseria species and no homologues have yet been identified in other species.

References

1. ^ Mellin JR, Goswami S, Grogan S, Tjaden B, Genco CA (May 2007). "A novel fur- and iron-regulated small RNA, NrrF, is required for indirect fur-mediated regulation of the sdhA and sdhC genes in Neisseria meningitidis". J. Bacteriol. 189 (10): 3686–94. doi:10.1128/JB.01890-06. PMC 1913314. PMID 17351036. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1913314. 
2. ^ Metruccio MM, Fantappiè L, Serruto D, et al. (February 2009). "The Hfq-dependent small noncoding RNA NrrF directly mediates Fur-dependent positive regulation of succinate dehydrogenase in Neisseria meningitidis". J. Bacteriol. 191 (4): 1330–42. doi:10.1128/JB.00849-08. PMC 2631994. PMID 19060140. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2631994. 

External links

• Rfam entry for NrrF RNA